MiR-199a-3p suppresses proliferation and invasion of prostate cancer cells by targeting Smad1
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Feng Qu1,2,*, Jinyu Zheng3,*, Weidong Gan1,2,*, Huibo Lian1,2, Hua He4, Wuping Li4,5, Tian Yuan4, Yaling Yang4, Xiaogong Li1, Changwei Ji1,2, Xiang Yan1,2, Linfeng Xu1,2 and Hongqian Guo1,2
1Department of Urology, The Affiliated Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, 210008, China
2Institute of Urology, Nanjing University, Nanjing, Jiangsu, 210093, China
3Department of Pathology, The Affiliated Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, 210008, China
4Department of Hematopathology, Division of Pathology and Laboratory Medicine, University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA
5Department of Lymphoma, Jiangxi Cancer Hospital, Nanchang, Jiangxi, 330029, China
*These authors contributed equally to this work
Feng Qu, email: email@example.com
Hongqian Guo, email: firstname.lastname@example.org
Keywords: MiR-199a-3p, Smad1, prostate cancer, proliferation, invasion
Received: February 10, 2017 Accepted: March 10, 2017 Published: April 18, 2017
Objectives: This study was intended to analyze effects of miR-199a-3p and Smad1 on proliferation, migration and invasion of prostate cancer (PCa) cells.
Results: MiR-199a-3p was significantly decreased in PCa tissues in comparison to that in adjacent normal tissues (P < 0.05). Over-expressed miR-199a-3p markedly suppressed proliferation and invasion of PCa cells (P < 0.05). MiR-199a-3p was negatively correlated with Smad1 expression, and overexpression of Smad1 could antagonize the effects of miR-199a-3p on PCa cells.
Materials and methods: The PCa tissues and their adjacent normal tissues were collected from 54 PCa patients. Expressions of miR-199a-3p and Smad1 mRNA in tissues and cells were evaluated with real-time quantitative polymerase chain reaction (RT-qPCR), and immunohistochemistry assay was used to detect Smad1 protein expressions. The target relationship between miR-199a-3p and Smad1 was assessed by luciferase reporter assay. The PCa cell lines (i.e. PC-3 cells) were transfected with miR-199a-3p mimics and Smad1-cDNA. MTT and Transwell assays were applied to detect proliferative, migratory and invasive abilities of PCa cells.
Conclusions: MiR-199a-3p suppressed proliferation and invasion of PCa cells by targeting Smad1.
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