Oncotarget

Clinical Research Papers:

Expression, prognosis and functional role of Thsd7a in esophageal squamous cell carcinoma of Kazakh patients, Xinjiang

Zhichao Hou, Abulajiang Abudureheman, Lei Wang, Ayshamgul Hasim, Julaiti Ainiwaer, Haiping Zhang, Madiniyat Niyaz, Halmurat Upur and Ilyar Sheyhidin _

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Oncotarget. 2017; 8:60539-60557. https://doi.org/10.18632/oncotarget.16966

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Abstract

Zhichao Hou1, Abulajiang Abudureheman1, Lei Wang1, Ayshamgul Hasim2, Julaiti Ainiwaer1, Haiping Zhang1, Madiniyat Niyaz3, Halmurat Upur4 and Ilyar Sheyhidin1

1 Department of Thoracic Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region, PR China

2 Department of Pathology of Medical University of Xinjiang, Urumqi, Xinjiang Uyghur Autonomous Region, PR China

3 Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region, PR China

4 Department of Uyghur Medicine, Xinjiang Medical University, Urumqi, PR China

Correspondence to:

Ilyar Sheyhidin, email:

Keywords: esophageal squamous cell carcinoma, Thsd7a, Kazakh patients, prognosis, function

Received: December 22, 2016 Accepted: March 01, 2017 Published: April 08, 2017

Abstract

Thsd7a (Thrombospondin type 1 domain containing 7a) is a critical transmembrane protein. Studies have indicated that Thsd7a was associated with cytoskeletal organization, cell migration and filopodia formation. However, the involvement of Thsd7a remains elusive in human Esophageal Squamous Cell Carcinoma (ESCC). Consequently, immunohistochemistry and reverse transcription-polymerase chain reaction were utilized to study the correlation between the expression of Thsd7a and clinical-pathological characteristics. The influence of Thsd7a on apoptosis, cell proliferating activity, cell cycle, migratory and invasive capacity was determined in Eca 109 and EC 9706 cell lines in vitro. And the influence on proliferating activity was testified using naked mice model in vivo. In addition, the potential molecular mechanism was tested by microarray. It was discovered that there is a certain correlation between Thsd7a and the Kazakh ESCC. By knocking out Thsd7a, the invasion, migration and proliferation could be decreased. And it could also arrest the cell cycle at G1 phase and increase the apoptosis rate. It was further verified that Thsd7a had obvious effect on proliferation in naked mice with xenograft of Eca109 cells. Finally, it was uncovered by microarray analysis that a variety of tumor genes and pathways related to Thsd7a. Together, it was demonstrated that Thsd7a might have a certain degree of carcinogenesis in ESCC.


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