Oncotarget

Research Papers:

Urine miR-21-5p as a potential non-invasive biomarker for gastric cancer

Hsiao-Wei Kao, Chao-Yu Pan, Chun-Hung Lai, Chew-Wun Wu, Wen-Liang Fang, Kuo-Hung Huang and Wen-Chang Lin _

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Oncotarget. 2017; 8:56389-56397. https://doi.org/10.18632/oncotarget.16916

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Abstract

Hsiao-Wei Kao1,*, Chao-Yu Pan1,2,*, Chun-Hung Lai1, Chew-Wun Wu3, Wen-Liang Fang3, Kuo-Hung Huang3 and Wen-Chang Lin1,2

1Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan

2Institute of Biomedical Informatics, National Yang-Ming University, Taipei, Taiwan

3Department of Surgery, Veterans General Hospital and National Yang-Ming University, Taipei, Taiwan

*These authors have contributed equally to this work

Correspondence to:

Wen-Chang Lin, email: [email protected]

Keywords: microRNA, liquid biopsy, urine samples, gastric cancer

Received: December 08, 2016    Accepted: March 14, 2017    Published: April 07, 2017

ABSTRACT

Many reports have implicated that microRNAs involve in cancer development and progression, such as miR-155 in breast cancers and miR-196 in gastric cancers. Furthermore, microRNAs are more stable than typical protein-coding gene mRNAs in varieties of clinical samples including body fluids. This suggests that they are potentially valuable biomarkers for cancer monitoring. In this study, we have used urine samples of gastric cancer patients to demonstrate the feasibility of urine microRNAs for gastric cancer detection. Urine samples of gastric cancer patients were extracted for total RNA, which were examined for the expression of miR-21-5p using quantitative stem-loop PCR. Our results demonstrated that miR-21-5p could be detected in small amounts of urine samples with good stability, and the expression levels of miR-21-5p were reduced following surgical removal of gastric cancer tissues. These results implicate that urine miR-21-5p could be utilized as a novel non-invasive biomarker of gastric cancer detection and monitoring.


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