CD54+ rabbit adipose-derived stem cells overexpressing HIF-1α facilitate vascularized fat flap regeneration
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De-Quan Li1,*, Guan-Ming Lu2,*, Yi-Dan Liang3,*, Zhi-Jie Liang4, Min-Hong Huang4, Qi-Liu Peng3, Dong-Hua Zou5, Rong-He Gu6, Fang-Tian Xu7, Hui Gao7, Zhen-Dong Chen7, Guang-Yi Chi8, Zhong-Heng Wei2, Li Chen9 and Hong-Mian Li8
1Department of Mammary Glands Surgery, The Third Hospital of Nanchang City, Nanchang 330009, China
2Department of Glands Surgery, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China
3Central Laboratory of Medical Science, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning 530022, China
4Department of Mammary Glands Surgery, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning 530022, China
5Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning 530022, China
6Department of Orthopedic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning 530022, China
7Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China
8Department of Plastic and Aesthetic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning 530022, China
9Department of Neurology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China
*These authors contributed equally to this work
Hong-Mian Li, email: firstname.lastname@example.org
Keywords: rabbit adipose-derived stem cells, HIF-1α, adipogenic differentiation, engineered fat flaps, angiogenesis
Received: January 31, 2017 Accepted: March 22, 2017 Published: April 01, 2017
Fat flap transplantation is frequently performed in patients suffering from soft tissue defects resulting from disease or trauma. This study explored the feasibility of constructing vascularized fat flaps using rabbit adipose-derived stem cells (rASCs) and collagen scaffolds in a rabbit model. We evaluated rASCs proliferation, paracrine function, adipogenesis, vascularization, and CD54 expression, with or without HIF-1α transfection in vitro and in vivo. We observed that adipogenic differentiation potential was greater in rASCs with high CD54 expression (CD54+rASCs) than in those with low expression (CD54–rASCs), both in vitro and in vivo. HIF-1α overexpression not only augmented this effect, but also enhanced cell proliferation and paracrine function in vitro. We also demonstrated that HIF-1α-transfected CD54+rASCs showed enhanced paracrine function and adipogenic capacity, and that paracrine function increases expression of angiogenesis-related markers. Thus, CD54+rASCs overexpressing HIF-1α enhanced large volume vascularized fat flap regeneration in rabbits, suggesting CD54 may be an ideal candidate marker for ASCs adipogenic differentiation.
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