6-Shogaol attenuates LPS-induced inflammation in BV2 microglia cells by activating PPAR-γ
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Qinghe Han2, Qinghai Yuan2 and Guanghong Xie1
1College of Veterinary Medicine, Jilin University, Changchun 130062, China
2The Second Hospital of Jilin University, Changchun 130000, China
Guanghong Xie, email: Xiegh@jlu.edu.cn
Keywords: 6-Shogaol, LPS, PPAR-γ, BV2 microglia
Received: November 08, 2016 Accepted: March 01, 2017 Published: March 30, 2017
6-Shogaol, a pungent agent isolated from Zingiber officinale Roscoe, has been known to have anti-tumor and anti-inflammatory effects. However, the anti-inflammatory effects and biological mechanism of 6-Shogaol in LPS-activated BV2 microglia remains largely unknown. In this study, we evaluated the anti-inflammatory effects of 6-Shogaol in LPS-activated BV2 microglia. 6-Shogaol was administrated 1 h before LPS treatment. The production of inflammatory mediators were detected by ELISA. The expression of NF-κB and PPAR-γ were detected by western blot analysis. Our results revealed that 6-Shogaol inhibited LPS-induced TNF-α, IL-1β, IL-6, and PGE2 production in a concentration dependent manner. Furthermore, 6-Shogaol inhibited LPS-induced NF-κB activation by inhibiting phosphorylation and nuclear translocation of NF-κB p65. In addition, 6-Shogaol could increase the expression of PPAR-γ. Moreover, inhibition of PPAR-γ by GW9662 could prevent the inhibition of 6-Shogaol on LPS-induced inflammatory mediator production. In conclusion, 6-Shogaol inhibits LPS-induced inflammation by activating PPAR-γ.
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