NDV-D90 suppresses growth of gastric cancer and cancer-related vascularization
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Hong Sui1,*, Kaibing Wang2,*, Rui Xie1,*, Xi Li3, Kunpeng Li3, Yuxian Bai1, Xishan Wang4, Bin Bai2, Dan Chen1, Jiazhuang Li1, Baozhong Shen5
1Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China
2Department of Intervention, The Second Hospital Affiliated Harbin Medical University, Harbin 150086, China
3Division of Swine Disease, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Medicine, Harbin 150069, China
4Department of Abdominal Surgery, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
5Department of Radiology, The Fourth Hospital of Harbin Medical University, Harbin 150001, China
*These authors contributed equally to this work and are joint First authors
Baozhong Shen, email: email@example.com
Hong Sui, email: firstname.lastname@example.org
Keywords: gastric cancer, newcastle disease viruses (NDV), apoptosis, vascular endothelial growth factor A (VEGF-A), matrix metallopeptidase 2 (MMP-2)
Received: March 01, 2017 Accepted: March 15, 2017 Published: March 25, 2017
Recent reports suggest promises on using oncolytic Newcastle disease viruses (NDV) to treat different cancers, while the effects of a NDV-D90 strain on gastric cancer remain unknown. Here we showed that NDV-D90 induced gastric cancer cell apoptosis in a dose-dependent manner in 3 gastric cancer cell lines BGC-823, SGC-7901 and MKN-28. Pronounced reduction in cell invasion was detected in NDV-D90-treated BGC-823 and SGC-7901 cells, but not in MKN-28 cells. The increases in cell apoptosis and reduction in cell growth in NDV-D90-treated gastric cancer cells seemingly resulted from augmentation of p38 signaling and suppression of ERK1/2 and Akt signaling. In vivo, orthotopic injection of NDV-D90 impaired tumor growth and induced intratumoral necrosis. Tumor cells that had been pre-treated with NDV-D90 showed defect in development of implanted tumor. Moreover, NDV-D90 appeared to reduce gastric tumor vascularization, possibly through suppression of vascular endothelial growth factor A and Matrix Metallopeptidase 2. Together, our data suggest that NDV-D90 may have potential anti-cancer effects on gastric cancer.
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