Oncotarget

Research Papers:

Effect on intensity of treadmill running on learning, memory and expressions of cell cycle-related proteins in rats with cerebral ischemia

Ya-Ning Zhao, Jian-Min Li _, Chang-Xiang Chen, Shu-Xing Li and Cheng-Jing Xue

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Oncotarget. 2017; 8:40633-40642. https://doi.org/10.18632/oncotarget.16537

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Abstract

Ya-Ning Zhao1, Jian-Min Li2, Chang-Xiang Chen1, Shu-Xing Li1 and Cheng-Jing Xue2

1Nursing and Rehabilitation College, North China University of Science and Technology, 063000, China

2The Neurosurgery of Affiliated Hospital, North China University of Science and Technology, 063000, China

Correspondence to:

Jian-Min Li, email: lijianmjm@126.com

Keywords: cerebral ischemia, treadmill running, learning and memory, cyclin A, cyclin E

Received: November 01, 2016    Accepted: February 22, 2017    Published: March 24, 2017

ABSTRACT

Objective: We discussed the intensity of treadmill running on learning, memory and expression of cell cycle-related proteins in rats with cerebral ischemia.

Method: Eighty healthy male SD rats were randomly divided into normal group, model group, intensity I group and intensity II group, with 20 rats in each group. The four-vessel occlusion method of Pulsinelli (4-VO) was used to induce global cerebral ischemia. Brain neuronal morphology was observed by hematoxylin-eosin (HE) staining at 3h, 6h, 24h and 48h after modeling, respectively. Hippocampal expressions of cyclin A and cyclin E were detected by immunohistochemistry. At 48h after modeling, the learning and memory performance of rats was tested by water maze experiment.

Result: Compared with the normal group, the other three groups had a significant reduction in surviving neurons, prolonging of escape latency and decreased number of passes over the former position of the platform (P<0.05). The number of surviving neurons and the number of passes over the former position of the platform were obviously lower in the model group than in intensity I group (P<0.05), but significantly higher compared with intensity II group (P<0.05). Escape latency of the model group was obviously prolonged as compared with intensity I group (P<0.05), but much shorter than that of intensity II group (P<0.05). Compared with the normal group, the expressions of cyclin A and cyclin E were significantly upregulated at different time points after modeling (P<0.05). The expression of the model group was higher than that of intensity I group, but lower than that of intensity II group (P<0.05).

Conclusion: Moderate intensity of treadmill running can help protect brain neurons and improve learning and memory performance of rats with global cerebral ischemia. But high intensity of treadmill running has a negative impact, possibly through the regulation of cell cycle-related proteins in ischemia/reperfusion injury.


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