Differential microRNA expression profiling in primary tumors and matched liver metastasis of patients with colorectal cancer
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Wenhua Li1,2,*, Jinjia Chang1,2,*, Duo Tong1,2, Junjie Peng2,3, Dan Huang2,4, Weijian Guo1,2, Wen Zhang1,2, Jin Li1,2,5
1Department of Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai, China
2Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
3Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
4Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, China
5East Hospital, Tongji University School of Medicine, Shanghai, China
*These authors contributed equally to this work
Jin Li, email: firstname.lastname@example.org
Keywords: colorectal carcinoma, microRNA, liver metastasis
Received: November 15, 2016 Accepted: February 28, 2017 Published: March 15, 2017
Background: Liver metastasis is common in patients with colorectal cancer (CRC), and is correlated with poor outcome. MicroRNAs (miRNAs) are small non-coding RNAs involved in cancer development and progression, but their role in CRC liver metastasis has not been extensively investigated.
Results: Thirteen miRNAs were deregulated in pCRCs compared to their matched liver metastases. Seventeen miRNAs were chosen for validation, which confirmed significantly reduced expression of miR-99b-5p, miR-377 and miR-200c and increased expression of miR-196b-5p in the tissue of liver metastasis. Furthermore, miR-200c and miR-196b-5p were positively correlated with shorter overall survival in pCRC patients with liver metastasis.
Materials and Methods: Firstly, affymetrix microarrays involving 1036 miRNAs were performed in two pairs of primary CRCs (pCRCs) and their matched liver metastases. Secondly, validation of the results was carried out on an independent cohort of 48 pairs of pCRCs and matched liver metastases using quantitative real-time polymerase chain reaction assay.
Conclusions: We discovered a pCRC liver metastasis-specific miRNA panel including miR-377, miR-99b-5p, miR-200c and miR-196b-5p through intensive validation. These miRNAs may function as prognostic factors in patients with metastatic CRC.
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