RMP promotes epithelial-mesenchymal transition through NF-κB/CSN2/Snail pathway in hepatocellular carcinoma
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Wei Zhou1,*, Qi Wang2,*, Yi Xu1, Jingting Jiang2, Jingchun Guo3, Huijun Yu1 and Wenxiang Wei1
1Department of Cell Biology, Institute of Bioengineering, School of Medicine, Soochow University, Suzhou 215123, China
2Department of Tumor Biotherapy, Third Affiliated Hospital of Soochow University, Changzhou 213003, China
3State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China
*These authors have contributed equally
Wenxiang Wei, email: [email protected]
Huijun Yu, email: [email protected]
Keywords: RMP, EMT, HCC, NF-κB, metastasis
Received: September 06, 2016 Accepted: February 20, 2017 Published: March 14, 2017
Epithelial-mesenchymal transition (EMT) is a significant risk factor for metastasis in hepatocellular carcinoma (HCC) patients and with poor prognosis. In this study, we demonstrate the key role of RPB5-mediating protein (RMP) in EMT of HCC cells and the mechanism by which RMP promote EMT. RMP increases migration, invasion, and the progress of EMT of HCC cells, which facilitates the accumulation of Snail, a transcriptional repressor involved in EMT initiation. NF-κB is activated by RMP, which directly promotes the expression of COP9 signalosome 2 (CSN2) to repress the degradation of Snail. Pulmonary metastases mouse model demonstrates that RMP induces metastasis in vivo. Immunohistochemical analysis of human HCC tissues confirms the correlation of RMP with the expression of E-cadherin, p65, CSN2 and Snail in vivo. Collectively, these findings indicate that RMP promotes EMT and HCC metastasis through NF-κB/CSN2/Snail pathway. These results suggest that RMP and p65 may serve as potential candidates of the targets in the treatment of metastatic HCC.
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