Oncotarget

Research Papers:

DNA methylation directly downregulates human cathelicidin antimicrobial peptide gene (CAMP) promoter activity

Xi Chen, Guangying Qi, Mingqun Qin, Yantao Zou, Kanghua Zhong, Ying Tang, Yong Guo, Xinxiang Jiang, Lihua Liang and Xianqiong Zou _

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Oncotarget. 2017; 8:27943-27952. https://doi.org/10.18632/oncotarget.15847

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Abstract

Xi Chen1,*, Guangying Qi2,4,*, Mingqun Qin3,*, Yantao Zou1, Kanghua Zhong1, Ying Tang1, Yong Guo1, Xinxiang Jiang3, Lihua Liang3, Xianqiong Zou1

1College of Biotechnology, Guilin Medical University, Guilin 541100, Guangxi, P. R. China

2Department of Pathology and Physiopathology, Guilin Medical University, Guilin 541004, Guangxi, P. R. China

3Department of Stomatology, Affiliated Hospital of Guilin Medical University, Guilin 541004, Guangxi, P. R. China

4Laboratory of Tumor Immunology and Microenvironmental Regulation, Guilin Medical University, Guilin 541004, Guangxi, P. R. China

*These authors contributed equally to this work

Correspondence to:

Xianqiong Zou, email: xianqiongzou2009@yahoo.com

Keywords: CAMP, LL-37, OSCC, DNA methylation, promoter

Received: September 01, 2016     Accepted: February 20, 2017     Published: March 02, 2017

ABSTRACT

LL-37, the active product of human cathelicidin antimicrobial peptide (CAMP) has a broad spectrum of antibacterial activity. LL-37 also has important physiological functions in immune regulation, angiogenesis and in modulating apoptosis. The roles of LL-37 in oral squamous cell carcinoma (OSCC) are still not clear. The correlation between DNA methylation and human CAMP expression is also unknown. Here human CAMP/LL-37 expression was assessed by immunohistochemistry in normal and OSCC tissues. The results indicated that low expression of CAMP/LL-37 correlated with histological differentiation and lymph node metastasis and also promoted tumor progression. A cell-specific methylation pattern in the promoter region of human CAMP was detected. Treatment with 5-aza-2’-deoxycytidine, a DNA demethylation reagent can increase human CAMP expression in epithelial cancer cells. The reporter assay showed that unmethylated human CAMP promoter activity was significantly higher than methylated promoter activity. Taken together, these results suggested that human CAMP/LL-37 might act as a tumor-suppressor in OSCC and DNA methylation might play roles during carcinogenesis via directly downregulating human CAMP promoter activity.


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