Oncotarget

Research Papers:

Anti-cancer effect of novel PAK1 inhibitor via induction of PUMA-mediated cell death and p21-mediated cell cycle arrest

Tae-Gyun Woo, Min-Ho Yoon, Shin-Deok Hong, Jiyun Choi, Nam-Chul Ha, Hokeun Sun and Bum-Joon Park _

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Oncotarget. 2017; 8:23690-23701. https://doi.org/10.18632/oncotarget.15783

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Abstract

Tae-Gyun Woo1, Min-Ho Yoon1, Shin-Deok Hong1, Jiyun Choi2, Nam-Chul Ha3, Hokeun Sun2, Bum-Joon Park1

1Department of Molecular Biology, Pusan National University, Busan, Republic of Korea

2Department of Statistics, Pusan National University, Busan, Republic of Korea

3College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea

Correspondence to:

Bum-Joon Park, email: bjpark1219@pusan.ac.kr

Keywords: PAK1, anti-cancer, p21, PUMA, Bcl-2

Received: June 20, 2016     Accepted: February 06, 2017     Published: February 28, 2017

ABSTRACT

Hyper-activation of PAK1 (p21-activated kinase 1) is frequently observed in human cancer and speculated as a target of novel anti-tumor drug. In previous, we also showed that PAK1 is highly activated in the Smad4-deficient condition and suppresses PUMA (p53 upregulated modulator of apoptosis) through direct binding and phosphorylation. On the basis of this result, we have tried to find novel PAK1-PUMA binding inhibitors. Through ELISA-based blind chemical library screening, we isolated single compound, IPP-14 (IPP; Inhibitor of PAK1-PUMA), which selectively blocks the PAK1-PUMA binding and also suppresses cell proliferation via PUMA-dependent manner. Indeed, in PUMA-deficient cells, this chemical did not show anti-proliferating effect. This chemical possessed very strong PAK1 inhibition activity that it suppressed BAD (Bcl-2-asoociated death promoter) phosphorylation and meta-phase arrest via Aurora kinase inactivation in lower concentration than that of previous PAK1 kinase, FRAX486 and AG879. Moreover, our chemical obviously induced p21/WAF1/CIP1 (Cyclin-dependent kinase inhibitor 1A) expression by releasing from Bcl-2 (B-cell lymphoma-2) and by inhibition of AKT-mediated p21 suppression. Considering our result, IPP-14 and its derivatives would be possible candidates for PAK1 and p21 induction targeted anti-cancer drug.


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