Oncotarget

Research Papers:

mTOR/autophagy pathway in the hippocampus of rats suffering intermittent hypoxia preconditioning and global cerebral ischemia-reperfusion

Ya-Ning Zhao _, Xiang-Fei Guo, Jian-Min Li, Chang-Xiang Chen, Shu-Xing Li and Cheng-Jing Xu

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Oncotarget. 2017; 8:23353-23359. https://doi.org/10.18632/oncotarget.15058

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Abstract

Ya-Ning Zhao1, Xiang-Fei Guo1, Jian-Min Li2, Chang-Xiang Chen1, Shu-Xing Li1, Cheng-Jing Xu2

1Nursing and Rehabilitation College, North China University of Science and Technology, 063000, China

2The Neurosurgery of Affiliated Hospital, North China University of Science and Technology, 063000, China

Correspondence to:

Jian-Min Li, email: lijianmjm7@163.com

Keywords: OSAHS-patterned hypoxia, cerebral ischemia-reperfusion, mTOR, autophagy

Received: November 04, 2016    Accepted: December 12, 2016    Published: February 03, 2017

ABSTRACT

We explored the role of mTOR/autophagy pathway in the aggravation of cerebral ischemia-reperfusion nerve injury caused by intermittent hypoxia. Eighty male wistar rats were divided into four groups by the random number method: sham operation group (SO group, n=20), cerebral ischemia-reperfusion group (I/R group, n=20), intermittent hypoxia and cerebral ischemia-reperfusion group (IH+I/R group, n=20), intermittent hypoxia and cerebral ischemia-reperfusion group plus mTOR inhibitor group (inhibitor group, n=20).The results showed that compared with the SO group, HE staining showed structural damage of neurons at each time point, the immunohistochemical assay showed an increasing number of mTOR and beclin1 immune-positive cells (P<0.05) and RT-PCR showed enhanced expression of mTOR and beclin1 protein in the I/R group (P<0.05). Compared with the I/R group, HE staining showed exacerbating structural damage of neurons at each time point, the immunohistochemical assay showed an increasing number of mTOR and beclin1 immune-positive cells (P<0.05) and RT-PCR showed enhanced expression of mTOR and beclin1 protein in the IH+I/R group (P<0.05). Compared with the IH+I/R group, HE staining showed remissive structural damage of neurons at each time point, the immunohistochemical assay showed a decreasing number of mTOR immune-positive cells and a rising number of beclin1immune-positive cells (P<0.05) and RT-PCR showed weakened expression of mTOR protein and enhanced expression of beclin1 protein in the inhibitor group (P<0.05). Thence, the present study indicated that intermittent hypoxia preconditioning can aggravate the nerve injury of the global cerebral ischemia-reperfusion model, and the mechanism is associated with the activation of mTOR/autophagy pathway.


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