Oncotarget

Research Papers:

Molecular profiling of metastatic colorectal tumors using next-generation sequencing: a single-institution experience

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Oncotarget. 2017; 8:42198-42213. https://doi.org/10.18632/oncotarget.15030

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Jun Gong, May Cho, Marvin Sy, Ravi Salgia and Marwan Fakih _

Abstract

Jun Gong1, May Cho1, Marvin Sy1, Ravi Salgia1 and Marwan Fakih1

1Department of Medical Oncology, City of Hope National Medical Center, Duarte, CA, USA

Correspondence to:

Marwan Fakih, email: [email protected]

Keywords: metastatic colorectal cancer, comprehensive genomic profiling, next-generation sequencing, FoundationOne, retrospective

Received: September 06, 2016     Accepted: January 16, 2017     Published: February 02, 2017

ABSTRACT

Background: Recent molecular characterization of colorectal tumors has identified several molecular alterations of interest that are considered targetable in metastatic colorectal cancer (mCRC).

Methods: We conducted a single-institution, retrospective study based on comprehensive genomic profiling of tumors from 138 patients with mCRC using next-generation sequencing (NGS) via FoundationOne.

Results: Overall, RAS mutations were present in 51.4% and RAF mutations were seen in 7.2% of mCRC patients. We found a novel KRASR68S1 mutation associated with an aggressive phenotype. RAS amplifications (1.4% KRAS and 0.7% NRAS), MET amplifications (2.2%), BRAFL597Ralterations (0.7%), ARAFS214F alterations (0.7%), and concurrent RAS+RAF (1.4%), BRAF+RAF1 (0.7%), and rare PTEN-PIK3CA-AKT pathway mutations were identified and predominantly associated with poor prognosis. ERBB2 (HER2) amplified tumors were identified in 5.1% and all arose from the rectosigmoid colon. Three cases (2.2%) were associated with a hypermutated profile that was corroborated with findings of high tumor mutational burden (TMB): 2 cases with MSI-H and 1 case with a POLE mutation.

Conclusions: Comprehensive genomic profiling can uncover alterations beyond the well-characterized RAS/RAF mutations associated with anti-EGFR resistance. ERBB2 amplified tumors commonly originate from the rectosigmoid colon, are predominantly RAS/BRAF wild-type, and may predict benefit to HER2-directed therapy. Hypermutant tumors or tumors with high TMB correlate with MSI-H status or POLE mutations and may predict a benefit from anti-PD-1 therapy.


INTRODUCTION

Colorectal cancer (CRC) remains the third leading cause of cancer death in both men and women in the United States with an estimated 134,490 new cases and 49,190 deaths in 2016 [1]. Recent advances in the treatment of metastatic CRC (mCRC) have identified improved outcomes with the addition of epidermal growth factor receptor (EGFR)-targeting agents to conventional combination cytotoxic therapy in patients with extended RAS wild-type tumors. In contrast, activating mutations in the RAS gene (KRAS or NRAS, present in approximately 50% of cases of mCRC) and BRAF gene (present in about 5% of mCRC patients) have been associated with lack of clinically meaningful benefit or harm when anti-EGFR therapy is employed [2]. The identification of candidates for anti-EGFR therapy through the exclusion of RAS and BRAF mutations in mCRC serves as a model of selecting optimal therapy based on patient genomic profiles and molecular phenotypes.

Several decades of genomic studies, including the use of more recent next-generation sequencing (NGS), have expedited the search of genetic alterations for potential therapeutic targeting in CRC [3, 4]. Recently, comprehensive molecular characterization of 224 colorectal tumors was performed by The Cancer Genome Atlas (TCGA) Network [5]. Sixteen percent of colorectal tumors were found to be hypermutated and more commonly found in the right colon with 75% of these cases demonstrating expectedly high microsatellite instability (MSI-H). Twenty-four genes were identified to have significant mutations of interest including APC, SMAD4, TP53, PIK3CA, and KRAS mutations, as expected. Interestingly, mutations, deletions, or amplifications of the ERRB gene family were found in 19% of tumors. In sum, this genomic analysis identified several molecular alterations that are considered targetable, including mediators of dysregulated WNT, RAS, and PI3K pathways such as ERRB2, ERRB3, MEK, AKT, MTOR, IGF2, and IGFR.

The recent identification of gene mutations and amplifications of potential significance for therapeutic purposes has led us to investigate the genomic profiles of mCRC patients using NGS (FoundationOne). Here, we describe a single-institution experience in reporting results from comprehensive genomic analysis of tumors from 138 mCRC patients. We aim to characterize genetic alterations present in our study population that have known correlates to prognosis, therapeutic resistance, and potential therapeutic targets in mCRC. In this study, we also report the existence of concurrent gene mutations rarely described in the literature and novel mutations and amplifications that can lead to targeting outside of National Comprehensive Cancer Network (NCCN) standard treatments.

RESULTS

Study population

The molecular results from FoundationOne testing of tumors from 138 mCRC patients are summarized in Table 1. The median age of our study group was 56 years (range 27-88) with 59.4% (82) males and 40.6% (56) females. The most common ethnicity was White (85, 61.6%) followed by Asian (29, 21.0%). The most common sites of primary were sigmoid colon (33.3%), rectum (19.6%), and cecum (15.2%). Sixty-eight patients (49.3%) had KRAS mutations, 9 patients (6.5%) had BRAF mutations, 3 (2.2%) had NRAS mutations, 1 (0.7%) had an ARAF mutation, 1 (0.7%) had a RAF-1 mutation, 7 (5.1%) had ERRB2 amplifications, 25 (18.1%) had PIK3CA mutations, 15 (10.9%) had PTEN mutations, 4 (2.9%) had AKT mutations, and 3 (2.2%) had MET amplifications.

Table 1: Demographics/patient characteristics

Characteristic (n = 138)

Frequency (%)

Age (at initial diagnosis)

Median (range)

56 (27-88)

Sex

 Male

82 (59.4%)

 Female

56 (40.6%)

Ethnicity

 White

85 (61.6%)

 Asian

29 (21.0%)

 Black

7 (5.1%)

 Other

3 (2.2%)

 Unknown

14 (10.1%)

Primary disease site

 Sigmoid colon

46 (33.3%)

 Rectal

27 (19.6%)

 Cecum

21 (15.2%)

 Rectosigmoid

13 (9.4%)

 Colon NOS

31 (22.5%)

Stage (at diagnosis)

 II

5 (3.6%)

 III

16 (11.6%)

 IV

117 (84.8%)

Relapsed disease

 Yes

52 (37.7%)

 No

86 (62.3%)

KRAS alterations

68 (49.3%)

 G12D

23 (16.7%)

 G13D

10 (7.2%)

 G12V

8 (5.8%)

 G12S

7 (5.1%)

 G12C

6 (4.3%)

 A146T

3 (2.2%)

 G12A

2 (1.4%)

 Amplification

2 (1.4%)

 Q61H

1 (0.7%)

 Q61K

1 (0.7%)

 Q61L

1 (0.7%)

 K117N

1 (0.7%)

 R68S

1 (0.7%)

 A146V

1 (0.7%)

 A146V^sub

1 (0.7%)

BRAF alterations

9 (6.5%)

 V600E

5 (3.6%)

 D594G

1 (0.7%)

 G466V

1 (0.7%)

 G469E

1 (0.7%)

 L597R

1 (0.7%)

NRAS alterations

 Q61K

2 (1.4%)

 Amplification

1 (0.7%)

ARAF mutation

 S214F

1 (0.7%)

RAF1 mutation

 S257L

1 (0.7%)

ERBB2 alterations

 Amplification

7 (5.1%)

MET alterations

 Amplification

3 (2.2%)

AKT1/AKT2 mutations

 AKT1

3 (2.2%)

 AKT2

1 (0.7%)

Total number of clinically significant alterations

Median (range) 5 (1-25)

MSI

 MSS

121 (87.7%)

 MSI-L

0 (0.0%)

 MSI-H

2 (1.4%)

 Unknown/not reported

15 (10.9%)

NOS: Not otherwise specified; MSI: Microsatellite instability; MSS: Microsatellite stable; MSI-L: MSI low; MSI-H: MSI high.

RAS mutations

Overall, RAS mutations were present in 51.4% of our mCRC patients, RAF mutations were seen in 7.2%, of which RAS+RAF concurrent mutations were seen in 1.4%. The remainder (42.8%) were RAS/RAF wild type (Figure 1). The most common RAS mutations were KRAS mutations of exon 2 (codons 12 and 13) including G12D (32.4%), G13D (14.1%), G12V (11.3%), G12S (9.9%), G12C (8.5%), and G12A (2.8%, Figure 2). Beyond the well-established point mutations in codons 12 and 13 of exon 2 of KRAS, we identified mutations in codon 61 of exon 3 (Q61H, 1.4%; Q61K, 1.4%; Q61L, 1.4%), codon 117 of exon 4 (K117N, 1.4%), and codon 146 of exon 4 (A146V, 1.4%; A146V^sub, 1.4%; A146T, 4.2%). Two mutations (2.8%) in codon 61 (exon 3) of NRAS were also detected. Altogether, these non-KRAS exon 2 mutations constitute 15.5% of RAS mutations.

Proportion of RAS, RAF, RAS+RAF mutations, and RAS/RAF wild type status identified by comprehensive genomic profiling.

Figure 1: Proportion of RAS, RAF, RAS+RAF mutations, and RAS/RAF wild type status identified by comprehensive genomic profiling. RAS+RAF mutations are not included in RAS or RAF percentages.

Proportion of RAS alterations identified by comprehensive genomic profiling.

Figure 2: Proportion of RAS alterations identified by comprehensive genomic profiling. Arrows denote common mutations of exon 2 (codon 12-13). Brackets denote panel of extended RAS mutations or novel RAS mutation.

In our patient population, 2 KRAS amplifications (2.8%) and 1 NRAS amplification (1.4%) were identified. One patient was a 51-year-old female with KRAS amplified rectal cancer with synchronous diffuse metastases (lung and liver). Her best overall response to standard first-line combination chemotherapy (5-fluorouracil (5-FU) and irinotecan or FOLFIRI) plus anti-EGFR therapy (panitumumab) was stable disease (SD) for 6 months. The other patient with KRAS amplification was a 51-year-old male diagnosed with right-sided colon cancer and synchronous metastases to the liver and peritoneum who had rapid progression on first- and second-line non-anti-EGFR based therapies. Our 74-year-old male patient with NRAS amplification presented with poorly differentiated rectosigmoid adenocarcinoma and synchronous diffuse metastases (liver, mesentery, and bones) and experienced progressive disease (PD) at 2 months on second-line FOLFIRI + cetuximab. Notably, a novel KRASR68S1 alteration (Figure 2) was identified in a 41-year-old female (1.4%) with rectal cancer and synchronous metastases to the liver and retroperitoneal and supraclavicular lymph nodes who experienced PD at 2 months on anti-EGFR therapy with second-line irinotecan + cetuximab.

RAF mutations

A total of 11 RAF mutations (1 concurrent BRAF+RAF1 mutation) were found in 7.2% of our patients (Figure 3). Of these, BRAFV600E activating mutations (exon 15) were the most common single mutations present (40.0%). One activating BRAFL597Ralteration (exon 15) was identified (10.0%) in a 56-year-old male with bulky rectal adenocarcinoma with synchronous metastases that progressed through 9 months of first-line anti-EGFR therapy. One activating ARAFS214F alteration was also identified (10.0%) in our series of RAF mutations. This 60-year-old male patient developed multiple recurrences of rectal adenocarcinoma including, most recently, metastatic disease to the lung treated with neoadjuvant 5-FU, oxaliplatin, and irinotecan (FOLFOXIRI) followed by metastatectomy; he remains in clinical remission. A dual BRAFV600E+KRASA164V^subalterationwas present (10.0%) in an elderly male (age 72) with poorly differentiated right-sided colon cancer with synchronous metastases on first-line systemic combination therapy without anti-EGFR agents. Here an oncogenic RAS alteration was paired with a known activating BRAF mutation.

Proportion of RAF alterations identified by comprehensive genomic profiling.

Figure 3: Proportion of RAF alterations identified by comprehensive genomic profiling. Arrows denote known activating mutations. Brackets denote known deactivating mutations.

Deactivating mutations in BRAFD594G(10.0%), BRAFG466V concurrent with KRASG12S (10.0%), and BRAFG469E concurrent with RAF1S257L (10.0%) were also identified. Our patient with a deactivating BRAFD594G mutation was a 59-year-old male with moderately-poorly differentiated right-sided colon cancer with diffuse metastases that was refractory to all standard of care chemotherapy, including FOLFIRI + cetuximab, and ultimately died from progressive disease. Interestingly, he was noted to have a concurrent MET amplification. The patient with a deactivating BRAFG466V mutation concurrent with an activating KRASG12S mutation was a 51-year-old male with right-sided colon cancer with diffuse metastases that progressed with carcinomatosis while on FOLFOX and immediately following salvage debulking surgery with hyperthermic chemotherapy. Notably, he currently has achieved ongoing partial response (PR) on third-line FOLFIRI and bevacizumab (41+ cycles). Our 66-year-old female with dual deactivating BRAFG469E and activating RAF1S257L mutation presented with a right colon cancer with synchronous metastases to bone, liver, lung, and peritoneum. Her disease was refractory to FOLFOX + bevacizumab and is currently on second-line FOLFIRI + bevacizumab with a clinical benefit.

ERBB2 amplifications

Seven patients (5.1%) were found to have ERRB2 amplified tumors with one having a concurrent KRASG12D mutation (Figure 4). The majority of these tumors were MSS (87.5%) with HER2 copy numbers that ranged from 9-190 (Table 2). Notably, all ERRB2 amplified tumors were located in the rectosigmoid colon as its primary disease site. Four patients with RAS wild-type ERBB2 amplification received anti-EGFR therapy, 3 experienced SD ≥ 4 months (2 first-line and 1 second-line) and 1 (second-line) experienced a PR lasting for 5 months as their best overall response to anti-EGFR therapy. The concurrent ERRB2 amplified and KRASG12D mutated tumor was found in a 58-year-old male with moderately differentiated rectal adenocarcinoma with synchronous solitary liver metastasis treated with neoadjuvant 5-FU, oxaliplatin (FOLFOX) followed by hepatic resection and resection of primary – he is currently under surveillance and without evidence of disease.

Proportion of ERBB2 amplifications identified by comprehensive genomic profiling.

Figure 4: Proportion of ERBB2 amplifications identified by comprehensive genomic profiling.

Table 2: Subset of ERBB2 amplifications

Copy number

Dual mutations

Age

Race/sex

Primary disease site

Lines of systemic therapy

Best overall response to anti-EGFR

MSI

9

55

Latino/M

Rectum

2

SD (4 mo)

NR

12

88

Middle Eastern/M

Sigmoid

1

MSS

37

50

White/F

Rectum

2

MSS

77

KRAS G12D

58

White/M

Rectum

2

MSS

93

60

White/M

Rectosigmoid

2

PR (5 mo)

MSS

189

48

White/M

Sigmoid

4

SD (4 mo)

MSS

190

71

Asian/M

Sigmoid

2

SD (5 mo)

MSS

EGFR: Epidermal growth factor receptor; MSI: Microsatellite instability; SD: Stable disease; NR: Not reported; MSS: Microsatellite stable; PR: Partial response.

AKT1/2 mutations

Three patients (2.2%) had AKT1E17K mutations while 1 patient (0.7%) had an AKT2E17K mutation (Table 3). Of these, a majority had concurrent mutations (75%) and tumors located in the right colon (75%). One AKT1E17K mutated tumor was found to have concurrent BRAFV600E +KRASA164V^subalterations with phenotype described above. One AKT1E17K mutated tumor had concurrent alterations in KRASA146T+PIK3CAG106V and was found in a 61-year-old male with initial right-sided colon cancer that recurred with metastases to the liver showing moderately differentiated colon adenocarcinoma. His tumor was characterized by aggressive features, including metastatic disease recurrence following a diagnosis of stage I disease, and development of bony metastases within the first year of recurrence. A concurrent AKT2E17K+KRASG12C altered tumor was found in a 57-year-old female with originally moderately differentiated sigmoid adenocarcinoma that was resected but recurred with metastases to the retroperitoneal lymph nodes currently on first-line FOLFIRI + bevacizumab.

Table 3: Subset of AKT1/2 mutations

Variant

Dual mutations*

Age

Race/Sex

Primary disease site

Lines of systemic therapy

MSI

E17K (AKT1)

KRAS A146T

61

Black/M

Right colon, NOS

2

NR

E17K (AKT1)

BRAF V600E, KRAS A146^sub

72

White/M

Ascending colon

1

MSS

E17K (AKT1)

69

White/F

Ascending colon

1

MSS

E17K (AKT2)

KRAS G12C

57

Asian/F

Sigmoid

1

MSS

*For concurrent RAS and BRAF mutations only, NOS: Not otherwise specified; MSI: Microsatellite instability; NR: Not reported; MSS: Microsatellite stable.

PIK3CA and PTEN mutations

In total, we identified 25 patients (18.1%) with PIK3CA alterations in our cohort (Table 4). The most common primary disease sites included cecum (36.0%), sigmoid colon (12.0%), and rectum (12.0%). Notably, right-sided colon cancers comprised nearly half (48.0%) of tumors with PIK3CA alterations. The most commonly identified variants were E545K (24.0%, exon 9), E542K (12.0% exon 9), E110del (8.0%), and Q546K (8.0%). Tumors with PIK3CA alterations frequently had concurrent mutations in the RAS-RAF-MAPK signaling pathway. A majority (19 or 76.0%) had concurrent mutations in KRAS (G12D 36.0%, G12S 12.0%, G13D 8.0%, and A146T 8.0%). Two patients (8.0%) with PIK3CA tumors were found to have concurrent deactivating BRAF mutations (G466V and G469E). Notably, these 2 patients had additional alterations in KRASG12S and RAF1S257L, respectively, with phenotypes described above. We also identified additional alterations in the PTEN-PIK3CA-AKT signaling pathway in our group of PIK3CA altered tumors (Figure 5). Five patients (20.0%) with PIK3CA altered tumors also had PTEN mutations, while 1 patient (4.0%) had a dual PIK3CA and AKT1 mutated tumor. Of note, 1 female patient (age 55) with a dual PIK3CA and PTEN mutation had a rectal tumor demonstrating MSI-H and developed a solitary liver metastasis that has since been resected and treated with adjuvant FOLFOX – she is currently in remission.

Proportion of PIK3CA, PTEN, AKT1/2 mutations identified by comprehensive genomic profiling with overlap.

Figure 5: Proportion of PIK3CA, PTEN, AKT1/2 mutations identified by comprehensive genomic profiling with overlap. Values in parentheses represent numbers and not percentages.

Table 4: Subset of PIK3CA alterations

Characteristic (n = 25)

Frequency (%)

Age (at initial diagnosis)

Median (range) 55 (30-81)

Sex

 Male

14 (56.0%)

 Female

11 (44.0%)

Ethnicity

 White

17 (68.0%)

 Asian

2 (8.0%)

 Black

2 (8.0%)

 Other/unknown

4 (16.0%)

Primary disease site

 Right colon

12 (48.0%)

 Transverse colon

2 (8.0%)

 Left colon (includes sigmoid)

8 (32.0%)

 Rectum

3 (12.0%)

Variant

 E545K (exon 9)

6 (24.0%)

 E542K (exon 9)

3 (12.0%)

 E110del

2 (8.0%)

 Q546K

2 (8.0%)

 C901F

1 (4.0%)

 C420R

1 (4.0%)

 C420R+E726K

1 (4.0%)

 E545G^sub+R108H^sub

1 (4.0%)

 G106V

1 (4.0%)

 H1047L (exon 20)

1 (4.0%)

 H1047Y (exon 20)

1 (4.0%)

 N107del

1 (4.0%)

 N345K

1 (4.0%)

 P104L

1 (4.0%)

 P104_V105del

1 (4.0%)

 R88Q

1 (4.0%)

Concurrent KRAS mutations

19 (76.0%)

 G12D

9 (36.0%)

 G12S

3 (12.0%)

 G13D

2 (8.0%)

 A146T

2 (8.0%)

 G12V

1 (4.0%)

 G12C

1 (4.0%)

 A146V

1 (4.0%)

Concurrent PTEN mutations

5 (20.0%)

 H93Y^sub

1 (4.0%)

 L316fs*7

1 (4.0%)

 L57fs*6+N323fs*2

1 (4.0%)

 R130Q+R142W

1 (4.0%)

 Splice site 1008_1026+5del24

1 (4.0%)

Concurrent BRAF mutations

 G466V

1 (4.0%)

 G469E

1 (4.0%)

Concurrent AKT1 mutation

 E17K

1 (4.0%)

MSI

 MSS

21 (84.0%)

 MSI-L

0 (0.0%)

 MSI-H

1 (4.0%)

 Unknown/Not reported

3 (12.0%)

MSI: Microsatellite instability; MSS: Microsatellite stable; MSI-L: MSI low; MSI-H: MSI high.

MET amplifications

Three patients (2.2%) in our series had MET amplifications (Table 5). Two-thirds of these tumors were MSS, located in the right colon, and associated with concurrent mutations in RAS or RAF genes. One 67-year-old male was initially diagnosed with right-sided colon cancer (KRASG13D+MET alterations present) and synchronous liver metastases. His course has been punctuated by recurrent metastases to the liver and lungs despite several systemic and regional therapies. Another right-sided colon cancer was identified with both a deactivating BRAFD594G mutation and MET amplification with aggressive phenotype described above. A third patient was a 27-year-old male with primary rectal adenocarcinoma that recurred with metastases to the liver and retroperitoneal lymph nodes and refractory to capecitabine + irinotecan + cetuximab. In particular, 2 of 3 patients with MET amplications and RAS/BRAFV600E wild-type tumors were refractory to anti-EGFR-based therapies.

Table 5: Subset of MET amplifications

Variant

Dual Mutations*

Age

Race/Sex

Smoker

Primary disease site

Lines of systemic therapy

MSI

MET

BRAF D594G

59

Asian/M

Yes

Right colon, NOS

4

MSS

MET

27

White/M

Yes

Rectum

2

NR

MET

KRAS G13D

67

Asian/M

No

Ascending colon

1

MSS

*For concurrent RAS and BRAF mutations only, MSI: Microsatellite instability; NOS: Not otherwise specified; NR: Not reported; MSS: Microsatellite stable.

Hypermutant status

The majority of our 138 patients with mCRC had tumors with <9 clinically significant alterations (121 or 87.7%) as described by FoundationOne reports (Table 6). The majority of these tumors were located in the left colon and all were MSS. Fourteen patients (10.1%) had 9-16 total alterations while only 3 patients (2.2%) were allocated to the highest number of clinically significant alterations category (17-25). Notably, 2 patients with MSI-H tumors were identified in the highest number of alterations group. One 55-year-old female patient was found to have a dual PIK3CA and PTEN mutated rectal tumor with phenotype described previously. Tumor mutational burden (TMB) from FoundationOne report showed a high TMB of 33 mutations per megabase (Mb). The other was a 47-year-old female with KRASG12V mutated metastatic rectal cancer that has progressed through 3 lines of systemic therapy and currently on anti-PD-1 therapy with pembrolizumab with a clinical response. Again, TMB corroborated her findings of a relatively hypermutated tumor with a TMB of 31 mutations/Mb. The third patient with a hypermutant FoundationOne profile had a MSS tumor with an associated POLEV411L mutation. Interestingly, this patient was elderly (age 80), had a right colon tumor, and had a recurrence pattern consistent with locoregional recurrence. This patient demonstrated a TMB of 122 mutations/Mb, which was the highest among the cohort.

Table 6: Total number of clinically significant alterations

No. of alterations

Frequency (%)

Age

Sex

Race

Primary disease site

MSI

<9

121 (87.7%)

Median (range)
56 (27-84)

F 48 (39.7%)
M 73 (60.3%)

White 74 (61.2%)
Asian 25 (20.7%)
Black 6 (4.9%)
Other 16 (13.2%)

Right colon 28 (23.1%)
Transverse colon 5 (4.1%)
Left colon (includes sigmoid) 60 (49.6%)
Rectum 22 (18.2%)
Colon, NOS 6 (5.0%)

MSS 121 (100%)

9-16

14 (10.1%)

Median (range)
60 (39-88)

F 6 (42.9%)
M 8 57.1%)

White 8 (57.1%)
Asian 4 (28.7%)
Black 1 (7.1%)
Unknown 1 (7.1%)

Right colon 6 (42.9%)
Left colon (includes sigmoid) 5 (35.7%)
Rectum 3 (21.4%)

MSS 13 (92.9%)
NR 1 (7.1%)

17-25

3 (2.2%)

Mean 59.7

F 2 (66.6%)
M 1 (33.3%)

White 3 (100%)

Rectum 2 (66.6%)
Right colon 1 (33.3%)

MSI-H 2 (66.6%)
MSS 1 (33.3%)

MSI: Microsatellite instability; NOS: Not otherwise specified; MSS: Microsatellite stable; NR: Not reported; MSI-H: MSI high.

DISCUSSION

Comprehensive molecular characterization of 138 tumors from patients with mCRC was performed via NGS (FoundationOne) in this single-institution retrospective study. Overall, 51.4% and 7.2% of our patients with mCRC were shown to carry RAS and RAF mutations, respectively, which is concordant with frequencies historically reported in mCRC [2]. The majority of our RAS mutations were KRAS mutations of exon 2 (codons 12 and 13), which represent those identified in initial phase III trials that predicted lack of benefit from anti-EGFR therapy in mCRC [6, 7]. We also found that 15.5% of all RAS mutations in our population comprised a panel of extended RAS mutations. This is also consistent with recent data from the PRIME and CRYSTAL clinical trials, where exon 3 and 4 KRAS and exons 2, 3, and 4 NRAS mutations reflected 14-17% of RAS mutations [8, 9]. Identifying these rare RAS mutations has major clinical significance, given their association with anti-EGFR resistance [10].

Notably, we identified 2 KRAS amplifications and 1 NRAS amplification that are extremely rare and poorly characterized. These were found in 3 patients with diffusely metastatic CRC progressive through several lines of systemic therapy including anti-EGFR therapy.

Putative high-level amplifications of NRAS were observed in <1% of cases in TCGA dataset though its significance in CRC remains poorly described [5]. KRAS amplifications have been associated with acquired resistance to EGFR inhibitors cetuximab or panitumumab in CRC preclinical models [11]. To our knowledge, we are the first to report a novel KRASR68S1 alteration that was associated with a particularly aggressive phenotype and PD at 2 months on anti-EGFR therapy with cetuximab.

The majority of RAF mutations found in our population were BRAFV600E activating mutations (exon 15), which have been historically associated with poorer survival, resistance to chemotherapy, and lack of clinical benefit with anti-EGFR therapy in mCRC [1215]. We also identified a lone BRAFL597Ralteration (exon 15), which is poorly described in CRC but has been shown to similarly activate RAF-MEK-ERK signaling in melanoma in vitro [16]. Of note, this patient received 9 months of first-line anti-EGFR therapy though our sample size of 1 precludes any meaningful generalizations. One ARAFS214F alteration was also identified in a patient whose course has been characterized by multiple recurrences of rectal cancer. Mutations in ARAF have been linked as oncogenic drivers in lung adenocarcinoma, and are exceedingly rare in CRC and comprise approximately 2% of cases in the CRC dataset from TCGA [5, 17]. Treatment with the oral RAF inhibitor, sorafenib, has demonstrated prolonged response in a case of refractory non-small-cell lung cancer and rapid responses in patients with refractory histiocytic neoplasms bearing somatic ARAF mutations [17, 18].

Despite a previous conception that KRAS and BRAF mutations are mutually exclusive, we found 1 dual BRAFV600E+KRASA164V^submutated tumor that, in our case, was associated with poor prognostic features [19]. One case of concurrent BRAFG466V+KRASG12S mutation and one patient with a concurrent BRAFG469E+RAF1S257L mutation were present in our cohort. BRAF mutants G466V and G469E have been shown to represent variants with impaired or complete loss of kinase activity in vitro [20, 21]. Nevertheless, it has been shown that tumorigenesis is promoted in the presence of deactivating BRAF mutations through oncogenic RAS mutation and/or CRAF (or RAF-1) signaling [21, 22]. In our study, one deactivating BRAFG466V mutation was paired with an oncogenic KRASG12S mutation, and one deactivating BRAFG469E mutation was paired with an oncogenic RAF1S257L alteration, supporting the notion of an evolutionary adaptation in the cancer genome to overcome BRAF mutations with impaired function. In both cases, there were associated features of poor prognosis though the dual BRAFG466V+KRASG12S mutated tumor has seen disease control recently on 41 cycles of FOLFIRI and bevacizumab, which may argue for varying degrees of relative contribution from each mutation on tumor phenotype. Interestingly, one patient with deactivating BRAFD594Gmutation was refractory to all lines of treatment, including anti-EGFR therapy, and ultimately died of aggressive disease. This is at odds with recent reports suggesting that BRAFD594G mutation may be an indicator of good prognosis [23]. It is unclear whether this patient’s concurrent MET amplification may have contributed to his overall poor prognosis and therapeutic resistance.

ERBB2 (HER2/neu) amplifications were found in 5.1% of our mCRC patients with the majority in KRAS wild-type tumors (except for 1 with a concurrent ERRB2+KRASG12D alteration). Another FoundationOne analysis of >10,000 cases of gastrointestinal malignancies identified HER2 amplifications and mutations in 3.0% and 4.8%, respectively, of cases from the CRC cohort [24]. Our patients with HER2 amplified tumors appeared to have shortened clinical benefit with anti-EGFR therapy, which is consistent with the recent phase II HERACLES trial where none of the patients with HER2 amplified, RAS/BRAF wild-type metastatic colorectal tumors had a response to anti-EGFR therapy [25]. Similar to the preponderance of left colon primary tumors in the HERACLES trial, all of our HER2 amplified tumors were located in the rectosigmoid colon. In short, the identification of HER2 amplifications in patients with RAS/BRAF wild-type metastatic colorectal tumors is of major significance given the clinical benefit derived from dual HER2-directed therapy including trastuzumab + lapatinib (HERACLES) or trastuzumab + pertuzumab (MyPathway) [25, 26].

PIK3CA, PTEN, and AKT mutations were identified in 18.1% (25), 10.9% (15), and 2.9% (4) of our mCRC patients, respectively. Many of these patients had metastatic tumors associated with aggressive features. In addition, 75% of AKT mutated tumors were located in the right colon, almost half (48.0%) of PIK3CA mutated tumors were right-sided colon cancers, and concurrent mutations in RAS-RAF-MAPK or PTEN-PIK3CA-AKT signaling were common. For example, 19 patients (76.0%) with PIK3CA mutations also had concurrent KRAS mutations while 5 (20.0%) and 1 (4.0%) with PIK3CA altered tumors also had concurrent PTEN and AKT1 mutations, respectively. Mutations in mediators of the PTEN-PIK3CA-AKT signaling pathway in CRC have been associated with poorer prognosis and lack of clinical response to anti-EGFR therapy [27, 28]. For PIK3CA mutations, in particular, prior studies have demonstrated that exon 9 mutations had no effect while exon 20 mutations were associated with resistance to anti-EGFR therapy [29]. However, this differential effect by exon has not been supported by recent meta-analysis [30]. Given the high rate of concurrent RAS mutations seen with PIK3CA and related pathway mutations, a definitive association between resistance to EGFR inhibition and PTEN-PIK3CA-AKT pathway mutations is difficult to make. Further studies are needed to resolve this issue.

Three patients (2.2%) demonstrated MET amplifications associated with poor prognostic features. MET amplification and increased c-MET expression have also been associated with an aggressive phenotype and therapeutic resistance, particularly to MEK inhibition, in mCRC [31, 32]. Interestingly, we have observed anti-EGFR refractoriness in 2 of our patients with MET amplifications despite the presence of a RAS- wild-type phenotype and lack of activating BRAF mutations. This is consistent with preclinical data suggesting MET activation as a mechanism of resistance to anti-EGFR therapy [33].

We lastly identified 3 patients (2.2%) with tumors categorized in the highest number of clinically significant alterations group (17-25) that also demonstrated high TMB as per FoundationOne. TMB categories per FoundationOne testing have been validated in melanoma patients treated with PD-1 blockade [34]. Response to PD-1 inhibitors was significantly superior in patients with high TMB (>23.1 mutations/MB) compared to intermediate or low TMB (3.2-23.1 mutations/MB and <3.2 mutations/MB, respectively). Furthermore, a recent phase II study showed that patients with advanced urothelial cancer who responded to the programmed death ligand 1 (PD-L1) inhibitor atezolizumab had a significantly higher TMB (median 12.4 mutations/Mb) than non-responders (median 6.4 mutations/Mb, p < 0.0001) [35]. Two patients had MSI-H tumors while 1 hypermutant tumor was MSS and harbored a POLE mutation. Interestingly, 42.9% of tumors with 9-16 clinically significant alterations were located in the right colon while one-third of tumors with 17-25 total alterations were located in the right colon; tumors with <9 number of alterations were predominantly located in the left colon. In the CRC dataset from TCGA, 75% of hypermutated tumors arose from the right colon yet not all of them were MSI-H [5]. Mutations in polymerase ε or POLE were found among 25% of hypermutated tumors in this cohort. Mutations in POLE have been shown to contribute to an ultramutated yet MSS phenotype in colorectal tumors [36]. A recent NGS study confirmed that increasing mutational load correlated with MSI yet colorectal tumors with the highest mutational burden that were distinct from MSI tumors all harbored POLE mutations [37]. Furthermore, mismatch repair-deficiency or MSI has recently been shown to predict clinical benefit to immune checkpoint blockade with anti-PD-1 therapy in mCRC [38]. The characterization of mutational load in CRC may serve as a better indicator than MSI status in determining a hypermutant profile that could predict benefit from immunotherapy. Our findings are hypothesis generating and offer support to consider molecular analysis of tumors to determine the total number of alterations as a potential correlate to MSI and candidacy for anti-PD-1 therapy in mCRC.

Future studies of larger size and, ideally, prospective design will be helpful in corroborating associations between molecular alterations of interest described in our study and prognosis, resistance to EGFR inhibition, and/or ability to be targeted for therapy in mCRC. Comparative genomic analyses have identified a high level of concordance particularly for RAS, BRAF, and PIK3CA mutations between colorectal primary and metastatic tumors [39, 40]. However, other molecular alterations may differ based on the site of tumor and/or exposure to chemotherapy [4144]. Although such mixed results are likely dependent on the specific mutation that is profiled, other factors including specimen integrity and sampling method may also contribute to heterogeneity. Indeed, further analyses are needed to describe the concordance or discordance of other mutations across tumor sites and treatment effects in mCRC, and careful consideration in design will be needed in order to account for confounding factors as described above.

In conclusion, comprehensive genomic profiling can uncover gene alterations beyond conventional RAS or RAF mutant subtypes that predict resistance to anti-EGFR therapy and in identifying potential therapeutic targets outside of NCCN standard treatments in mCRC. ERBB2 amplified tumors commonly originate from the rectosigmoid colon, are predominantly RAS/BRAF wild-type, and may predict benefit to HER2-directed therapy. Hypermutant tumors or tumors with POLE mutations may predict benefit to anti-PD-1 therapy. Our findings are hypothesis generating and warrant further investigation in larger datasets and in prospective settings.

MATERIALS AND METHODS

Study patients and tumor samples

Patients with advanced or metastatic (stage IV) colorectal cancer treated at the Gastrointestinal Medical Oncology Clinic at City of Hope National Medical Center (Duarte, CA) between April 2013 and February 2016 were screened for this study. Eligibility criteria was limited to those who underwent expanded genomic tumor analysis by FoundationOne. There were no exclusions to tumor histology, medical comorbidities, previous treatment or lines of prior therapy, or performance status. Comprehensive genomic profiling was conducted through NGS via FoundationOne (Foundation Medicine, Inc., Cambridge, MA) with reports generated from April 2013 to February 2016. The study was approved by the Institutional Review Board (IRB).

Next-generation sequencing

Comprehensive genomic analysis was conducted on tumor samples (formalin-fixed paraffin-embedded) retrieved from surgical resection, core needle biopsies, or excisional biopsies and delivered to Foundation Medicine, Inc. The NGS assay performed by FoundationOne has been previously described and validated [45]. The initial whole-genome shotgun library construction and hybridization-based capture of 4,557 exons from 287 cancer-related genes and 47 introns from 19 genes with frequent DNA rearrangements has since been expanded to identify genetic alterations across the coding regions of 315 cancer-related genes and introns from 28 genes commonly rearranged in solid cancers.

Study design

Retrospective analysis of genetic mutations, amplifications, or alterations present in our cohort of 138 patients with mCRC was performed through test results provided in an integrative report available via FoundationICE (Interactive Cancer Explorer). Patient demographics including age, sex, ethnicity, site of primary, stage at diagnosis, and number of previous treatments were obtained from chart abstraction of each patient’s electronic medical record (EMR). Microsatellite instability classified as stable (MSS), low (MSI-L), or high (MSI-H) were abstracted from pathology reports and response to anti-EGFR therapy, when available, was described according to Response Evaluation Criteria in Solid Tumors (RECIST) criteria and obtained from medical records [46]. The total number of clinically significant alterations for each patient was determined by tallying the sum of alterations included in the panel of clinically significant variants provided by FoundationICE reports and arbitrarily categorized into 3 groups (<9, 9-16, and > 16 total number of alterations). We defined hypermutant tumors as those in the highest number of mutations group that were also found to have high TMB as validated by FoundationOne (high >23.1 mutations/MB, intermediate 3.2-23.1 mutations/MB, and low <3.2 mutations/MB) [34].

Statistical analyses

All statistical analyses performed were descriptive and no formal statistical hypotheses were assessed. The sample size was determined by the total number of mCRC patients with FoundationOne results available. All descriptive statistics were conducted in Excel with associated formulas and functions.

CONFLICTS OF INTEREST

All authors declare no potential conflicts of interest.

GRANT SUPPORT

The research described was supported by the City of Hope Cancer Center Support Grant (CCSG) and CCSG-supported Core Facilities.

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