Research Papers:

Tumor suppressor miR-1 inhibits tumor growth and metastasis by simultaneously targeting multiple genes

Cuilian Liu _, Song Zhang, Qizhi Wang and Xiaobo Zhang

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Oncotarget. 2017; 8:42043-42060. https://doi.org/10.18632/oncotarget.14927

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Cuilian Liu1, Song Zhang1, Qizhi Wang2 and Xiaobo Zhang1

1College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou 310058, The People's Republic of China

2Department of Gastroenterology, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233030, The People's Republic of China

Correspondence to:

Xiaobo Zhang, email: [email protected]

Keywords: miR-1, target gene, tumor growth, metastasis

Abbreviations: TWF1: twinfilin actin binding protein 1; TMSB4X: thymosin beta 4, X-linked; WASF2: WAS protein family member 2; CNN3: calponin 3; CORO1C: coronin 1C

Received: October 15, 2016     Accepted: December 29, 2016     Published: January 31, 2017


Cancer progression depends on tumor growth and metastasis, which are activated or suppressed by multiple genes. An individual microRNA may target multiple genes, suggesting that a miRNA may suppress tumor growth and metastasis via simultaneously targeting different genes. However, thus far, this issue has not been explored. In the present study, the findings showed that miR-1 could simultaneously inhibit tumor growth and metastasis of gastric and breast cancers by targeting multiple genes. The results indicated that miR-1 was significantly downregulated in cancer tissues compared with normal tissues. The miR-1 overexpression led to cell cycle arrest in the G1 phase in gastric and breast cancer cells but not in normal cells. Furthermore, the miR-1 overexpression significantly inhibited the metastasis of gastric and breast cancer cells. An analysis of the underlying mechanism revealed that the simultaneous inhibition of tumor growth and metastasis mediated by miR-1 was due to the synchronous targeting of 6 miR-1 target genes encoding cyclin dependent kinase 4, twinfilin actin binding protein 1, calponin 3, coronin 1C, WAS protein family member 2 and thymosin beta 4, X-linked. In vivo assays demonstrated that miR-1 efficiently inhibited tumor growth and metastasis of gastric and breast cancers in nude mice. Therefore, our study contributed novel insights into the miR-1′s roles in tumorigenesis of gastric and breast cancers.

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