IL-17B activated mesenchymal stem cells enhance proliferation and migration of gastric cancer cells
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Qingli Bie1,*, Bin Zhang1,*, Caixia Sun2, Xiaoyun Ji1, Prince Amoah Barnie3, Chen Qi1, Jingjing Peng1, Danyi Zhang1, Dong Zheng1, Zhaoliang Su1, Shengjun Wang1,4, Huaxi Xu1,4
1Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China
2Department of Anesthesiology, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China
3Department of Biomedical and Forensic Sciences, School of Biological Sciences, University of Cape Coast, Cape Coast, Ghana
4Key Laboratory of Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China
*These authors contributed equally to this work
Huaxi Xu, email: [email protected]
Shengjun Wang, email: [email protected]
Keywords: gastric cancer, IL-17B, mesenchymal stem cells (MSCs), stemness
Received: November 21, 2016 Accepted: January 17, 2017 Published: January 27, 2017
Mesenchymal stem cells are important cells in tumor microenvironment. We have previously demonstrated that IL-17B/IL-17RB signal promoted progression of gastric cancer. In this study, we further explored the effect of IL-17B on mesenchymal stem cells in tumor microenvironment and its impact on the tumor progression. The results showed that IL-17B induced the expression of stemness-related genes Nanog, Sox2, and Oct4 in mesenchymal stem cells and enhanced its tumor-promoting effect. The supernatant from cultured mesenchymal stem cells after treating with exogenous rIL-17B promoted the proliferation and migration of MGC-803, therefor suggesting that rIL-17B might promote mesenchymal stem cells to produce soluble factors. In addition, rIL-17B also activated the NF-κΒ, STAT3, β-catenin pathway in mesenchymal stem cells. Our data revealed a new mechanism that IL-17B enhanced the progression of gastric cancer by activating mesenchymal stem cells.
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