Research Papers:

Identification of microRNAs implicated in the late differentiation stages of normal B cells suggests a central role for miRNA targets ZEB1 and TP53

Giorgio Malpeli _, Stefano Barbi, Simonetta Zupo, Gabriele Tosadori, Giovanni Scardoni, Anna Bertolaso, Silvia Sartoris, Stefano Ugel, Caterina Vicentini, Matteo Fassan, Annalisa Adamo, Mauro Krampera, Maria Teresa Scupoli, Carlo Maria Croce and Aldo Scarpa

PDF  |  HTML  |  Supplementary Files  |  How to cite

Oncotarget. 2017; 8:11809-11826. https://doi.org/10.18632/oncotarget.14683

Metrics: PDF 2036 views  |   HTML 2563 views  |   ?  


Giorgio Malpeli1,2, Stefano Barbi2, Simonetta Zupo3, Gabriele Tosadori4, Giovanni Scardoni4, Anna Bertolaso2, Silvia Sartoris5, Stefano Ugel5, Caterina Vicentini2,10, Matteo Fassan6, Annalisa Adamo7, Mauro Krampera7, Maria Teresa Scupoli8, Carlo Maria Croce9, Aldo Scarpa2,10

1Department of Surgical Sciences, Dentistry, Gynecology and Pediatrics, Section of Surgery, University of Verona, Verona, Italy

2Department of Diagnostics and Public Health, Section of Pathological Anatomy, University of Verona, Verona, Italy

3Laboratory of Molecular Diagnostics, IRCCS-AOU San Martino-IST, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy

4Center for BioMedical Computing (CBMC), University of Verona, Verona, Italy

5Department of Medicine, Section of Immunology, University of Verona, Verona, Italy

6Department of Medicine, Surgical Pathology and Cytopathology Unit, University of Padua, Padua, Italy

7Department of Medicine, Section of Hematology, Stem Cell Research Laboratory, University of Verona, Italy

8Department of Medicine, Section of Hematology, University of Verona, Italy

9Department of Molecular Virology, Immunology and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA

10Applied Research on Cancer-Network (ARC-NET), University of Verona, Verona, Italy

Correspondence to:

Giorgio Malpeli, email: [email protected]

Keywords: B cell development, follicle, germinal centre, microRNAs, network analysis

Received: June 17, 2016     Accepted: December 12, 2016     Published: January 17, 2017


In the late B cell differentiation stages, miRNAs expression modifications promoting or inhibiting key pathways are only partially defined. We isolated 29 CD19+ human B cell samples at different stages of differentiation: B cells from peripheral blood; naïve, germinal center (GC) and subepithelial (SE) B cells from tonsils. SE cells were further split in activated and resting B cell. The miRNA expression profile of these B cells was assessed by microarray analysis and selected miRNAs were validated by quantitative RT-PCR and in situ hybridization on normal tonsils. The comparison of all samples showed changes in 107 miRNAs in total. Among 48 miRNAs differentially expressed in naïve, GC and SE cells, we identified 8 miRNAs: mir-323, mir-138, mir-9*, mir-211, mir-149, mir-373, mir-135a and mir-184, strictly specific to follicular cells that had never been implicated before in late stages of B cell development. Moreover, we unveiled 34 miRNAs able to discriminate between CD5 activated B cells and resting B cells. The miRNAs profile of CD5 resting B cells showed a higher similarity to naïve CD5+ than CD5 activated B cells. Finally, network analysis on shortest paths connecting gene targets suggested ZEB1 and TP53 as key miRNA targets during the follicular differentiation pathway. These data confirm and extend our knowledge on the miRNAs-related regulatory pathways involved in the late B cell maturation.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 14683