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The SDF-1/CXCR4 axis promotes recovery after spinal cord injury by mediating bone marrow-derived from mesenchymal stem cells

Guo-Dong Wang, Yi-Xun Liu, Xiao Wang _, Yong-Le Zhang, Ya-Dong Zhang and Feng Xue

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Oncotarget. 2017; 8:11629-11640. https://doi.org/10.18632/oncotarget.14619

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Abstract

Guo-Dong Wang1,*, Yi-Xun Liu1,*, Xiao Wang1, Yong-Le Zhang1, Ya-Dong Zhang2, Feng Xue2

1Department of Orthopedic, Huaihe Hospital of Henan University, Kaifeng 475000, P.R. China

2Department of Orthopedic, Shanghai Fengxian Central Hospital, Branch of the Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 201400, P.R. China

*These are co-first authors

Correspondence to:

Xiao Wang, email: [email protected]

Keywords: bone marrow-derived mesenchymal stem cells, stromal cell-derived factor-l, chemotaxis cytokine receptor-4, spinal cord injury, hind limb motor function

Received: June 29, 2016     Accepted: December 16, 2016     Published: January 13, 2017

ABSTRACT

This study aims to explore the role of the SDF-1/CXCR4 axis in mediating BMSCs and SCI recovery. BMSCs were collected and SCI rat models were established. Wistar rats were assigned into the blank control, sham, SCI, SCI + BMSCs, SCI + BMSCs + SDF-1, SCI + BMSCs + AMD3100 (an inhibitor of SDF-1/CXCR4 axis) and SCI + BMSCs + SDF-1 + AMD3100 groups. Hind limb motor function was measured 7, 14, 21 and 28 days after operation. qRT-PCR, western blotting and ELISA was performed to determine the expressions of SDF-1, CXCR4, NGF, BDNF, GFAP and GAP-43, TNF-α, IL-1β, L-6 and IFN-γ. Hind limb motor function scores 7 days after the operation were reduced in the SCI rats of the blank control and sham groups. Hind limb function was found to be better in the SCI + BMSCs and SCI + BMSCs + SDF-1 groups than in the SCI, SCI + BMSCs + AMD3100 and SCI + BMSCs + SDF-1 + AMD3100 groups 14, 21 and 28 days after operation. Furthermore, the SCI group had lower SDF-1, CXCR4, NGF, BDNF and GAP-43 expressions but higher GFAP, TNF-α, IL-1β, IL-6 and IFN-γ than the blank control and sham groups 28 days after operation. While, the SCI + BMSCs, SCI + BMSCs + SDF-1 and SCI + BMSCs + SDF-1 + AMD3100 groups displayed opposite trends to the SCI and SCI + BMSCs + AMD3100 groups. In conclusion, SDF-1/CXCR4 axis promotes recovery after SCI by mediating BMSCs.


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