Direct analysis of aberrant glycosylation on haptoglobin in patients with gastric cancer
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Jae-Han Kim1, Sung Hyeon Lee2, Sookyung Choi2, Unyong Kim3, In Seok Yeo4, Su Hee Kim2, Myung Jin Oh3, Hantae Moon3, Jua Lee3, Seunghyup Jeong3, Min Gew Choi5, Jun Ho Lee5, Tae Sung Sohn5, Jae Moon Bae5, Sung Kim5, Yang Won Min5, Hyuk Lee5, Jun Haeng Lee5, Poong-Lyul Rhee5, Jae J. Kim5, Su Jin Lee5, Seung Tae Kim5, Jeeyun Lee5, Se Hoon Park5, Joon Oh Park5, Young Suk Park5, Ho Yeong Lim5, Won Ki Kang5, Hyun Joo An3, Jung Hoe Kim4
1Department of Food and Nutrition, Chungnam National University, Yuseong-Gu, Deajeon, Korea
2GLYCAN Co., Ltd., Healthcare Innovation Park, Bundang-Gu, Seongnam, Korea
3Graduate School of Analytical Science and Technology, Chungnam National University, Yuseong-Gu, Deajeon, Korea
4Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-Gu, Daejeon, Korea
5Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
Se Hoon Park, email: email@example.com
Keywords: stomach neoplasm, haptoglobin, N-glycan, glycoprotein, biomarker
Received: October 05, 2016 Accepted: December 21, 2016 Published: December 29, 2016
Based on our previous studies, differential analysis of N-glycan expression bound on serum haptoglobin reveals the quantitative variation on gastric cancer patients. In this prospective case-control study, we explore the clinically relevant glycan markers for gastric cancer diagnosis. Serum samples were collected from patients with gastric cancer (n = 44) and healthy control (n = 44). N-glycans alteration was monitored by intact analysis of Hp using liquid chromatography–mass spectrometry followed by immunoaffinity purification with the serum samples. Intensity and frequency markers were defined depending on the mass spectrometry data analysis. Multiple markers were found with high diagnostic efficacy. As intensity markers (I-marker), six markers were discovered with the AUC > 0.8. The high efficiency markers exhibited AUC of 0.93 with a specificity of 86% when the sensitivity was set to 95%. We additionally established frequency marker (f-marker) panels based on the tendency of high N-glycan expression. The AUC to conclude patients and control group were 0.82 and 0.79, respectively. This study suggested that N-glycan variation of serum haptoglobin were associated with patients with gastric cancer and might be a promising marker for the cancer screening.
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