Assessing the clinical value of microRNAs in formalin-fixed paraffin-embedded liposarcoma tissues: Overexpressed miR-155 is an indicator of poor prognosis
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Nikolaos Kapodistrias1, Konstantinos Mavridis2, Anna Batistatou3, Penelope Gogou4, Vasilios Karavasilis5, Ioannis Sainis1, Evangelos Briasoulis1, Andreas Scorilas2
1Cancer Biobank Center, University of Ioannina, University Campus, Ioannina, Greece
2Department of Biochemistry and Molecular Biology, National and Kapodistrian University of Athens, Panepistimiopolis, Athens, Greece
3Department of Pathology, School of Medicine, University of Ioannina, Greece
4Clinical Oncology Department, Norwich University Hospital, UK
5London Sarcoma Service, Department of Oncology, UCLH, London, UK
Andreas Scorilas, email: firstname.lastname@example.org
Keywords: liposarcoma, miRNAs, miR-155, miRNA expression normalization, prognostic biomarker
Received: July 05, 2016 Accepted: December 01, 2016 Published: December 28, 2016
Liposarcoma (LPS) is a malignancy with extreme heterogeneity and thus optimization towards personalizing patient prognosis and treatment is essential. Here, we evaluated miR-155, miR-21, miR-143, miR-145 and miR-451 that are implicated in LPS, as novel FFPE tissue biomarkers.
A total of 83 FFPE tissue specimens from primary LPS and lipomas (LPM) were analyzed. A proteinase K incubation-Trizol treatment coupled protocol was used for RNA isolation. After polyadenylation of total RNA and reverse transcription, expression analysis of 9 candidate reference and 5 target miRNAs was performed by qPCR. Genorm and NormFinder were used for finding the most suitable molecules for normalization. Survival analyses were performed in order to evaluate the prognostic potential of miRNAs.
MiR-103 and miR-191 are most suitable for normalization of miRNA expression in LPS. MiR-155 and miR-21 are clearly overexpressed (P<0.001) in LPS compared with LPM specimens, whereas miR-145 (P<0.001), miR-143 (P =0.008) and miR-451 (P=0.037) are underexpressed. MiR-155 (P=0.007) and miR-21 (P=0.029) are differentially expressed between well-differentiated, dedifferentiated, myxoid/round cell and pleomorphic LPs tumor subtypes. MiR-155 represents a novel independent indicator of unfavorable prognosis in LPS (HR = 2.97, 95% CI = 1.23–7.17, P = 0.016).
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