Research Papers:

Protective effects of resveratrol against mancozeb induced apoptosis damage in mouse oocytes

Yu LIU, Ya-Long WANG, Shu-wen HE, Ming-Huang CHEN, Zhen ZHANG, Xian-Pei FU, Bin-Bin FU, Bao-Qiong LIAO, Yan-Hong LIN, Zhong-Quan Qi and Hai-Long Wang _

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Oncotarget. 2017; 8:6233-6245. https://doi.org/10.18632/oncotarget.14056

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Yu Liu1,2, Ya-Long Wang1,2,*, Shu-wen He1,2,*, Ming-Huang Chen3, Zhen Zhang4, Xian-Pei Fu1,2, Bin-Bin Fu1,2, Bao-Qiong Liao1,2, Yan-Hong Lin5, Zhong-Quan Qi1,2, Hai-Long Wang1,2

1Organ Transplantation Institute, Medical College, Xiamen University, Xiamen City, Fujian Province, China

2Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen City, Fujian Province, China

3Department of Gynaecology and Obstetrics, Zhongshan Hospital, Xiamen University, Xiamen City, Fujian Province, China

4Xiamen Institute for Food and Drug Quality Control, Xiamen City, Fujian Province, China

5Department of Gynecology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China

*These authors contributed equally to this work

Correspondence to:

Hai-Long Wang, email: [email protected]

Zhong-Quan Qi, email: [email protected]

Keywords: oocyte, mancozeb, resveratrol, apoptosis

Received: September 06, 2016     Accepted: December 13, 2016     Published: December 21, 2016


Mancozeb, a mixture of ethylene-bis-dithiocarbamate manganese and zinc salts, is one of the most widely used fungicides in agriculture. Mancozeb could lead to mitochondria dysfunction, cellular anti-oxidation enzymes depletion and apoptotic pathways activation. Previous studies indicated the exposure of mancozeb through mother would lead to irregular estrous cycles, decreased progesterone levels, reduced litter sizes, and more frequent delivery of dead fetuses. In this study, we investigated mancozeb inducing reproductive toxicity, especially focusing on its apoptotic effect and epigenetic modifications. We also showed that resveratrol, a kind of phytoalexin found in peanuts and grapes, can alleviate mancozeb’s adverse effects, such as declined fertility, decreased ovary weight and primary follicles. Besides, mancozeb treated oocytes displayed suboptimal developmental competence and this can also be improved by treatment of resveratrol. More detailed investigation of these processes revealed that mancozeb increased reactive oxygen species, causing cell apoptosis and abnormal epigenetic modifications, and resveratrol can block these cytotoxic changes. Collectively, our results showed that resveratrol can alleviate mancozeb induced infertility and this was mainly through the correction of apoptotic tendency and the abnormity of cellular epigenetic modification.

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