Genome-wide analysis of differentially expressed lncRNAs and mRNAs in primary gonadotrophin adenomas by RNA-seq
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Jiye Li1, Chuzhong Li2, Jianpeng Wang3, Guidong Song1, Zheng Zhao1, Haoyuan Wang4, Wen Wang5, Hailong Li6, Zhenye Li5, Yazhou Miao1, Guilin Li1, Yazhuo Zhang2
1Beijing Neurosurgical Institute, Capital Medical University, Tiantan Xili, Dongcheng District, Beijing, China
2Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Beijing Institute for Brain Disorders Brain Tumor Center, China National Clinical Research Center for Neurological Diseases, Capital Medical University, Beijing, China
3Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China
4Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China
5Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
6Department of Neurosurgery, Navy General Hospital, Beijing, China
Yazhuo Zhang, email: [email protected]
Keywords: gonadotrophin adenoma, transcriptomics, ncRNA, pathway analysis, co-expression network
Received: November 09, 2016 Accepted: December 08, 2016 Published: December 15, 2016
Recently, long non-coding RNAs (lncRNAs) have received increased research interest owing to their participation via distinct mechanisms in the biological processes of nonfunctional pituitary adenomas. However, changes in the expression of lncRNAs in gonadotrophin adenoma, which is the most common nonfunctional pituitary adenomas, have not yet been reported. In this study, we performed a genome-wide analysis of lncRNAs and mRNAs obtained from gonadotrophin adenoma patients’ samples and normal pituitary tissues using RNA-seq. The differentially expressed lncRNAs and mRNAs were identified using fold-change filtering. We identified 839 lncRNAs and 1015 mRNAs as differentially expressed. Gene Ontology analysis indicated that the biological functions of differentially expressed mRNAs were related to transcription regulator activity and basic metabolic processes. Ingenuity Pathway Analysis was performed to identify 64 canonical pathways that were significantly enriched in the tumor samples. Furthermore, to investigate the potential regulatory roles of the differentially expressed lncRNAs on the mRNAs, we constructed general co-expression networks for 100 coding and 577 non-coding genes that showed significantly correlated expression patterns in tumor cohort. In particular, we built a special sub-network of co-expression involving 186 lncRNAs interacting with 15 key coding genes of the mTOR pathway, which might promote the pathogenesis of gonadotrophin tumor. This is the first study to explore the patterns of genome-wide lncRNAs expression and co-expression with mRNAs, which might contribute to the molecular pathogenesis of gonadotrophin adenoma.
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