Characterization and phylogenetic analysis of Krüppel-like transcription factor (KLF) gene family in tree shrews (Tupaia belangeri chinensis)
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Ming Shao1,2,*, Guang-Zhe Ge1,2,*, Wen-Jing Liu4,1,*, Ji Xiao4,1, Hou-Jun Xia1, Yu Fan1, Feng Zhao1, Bao-Li He3, Ceshi Chen1
1Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China
2Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China
3Department of Laboratory Animal Science, Kunming Medical University, Kunming, Yunnan 650500, China
4Medical Faculty, Kunming University of Science and Technology, Kunming, Yunnan, China
*These authors contributed equally to this work
Ceshi Chen, email: firstname.lastname@example.org
Keywords: KLF, tree shrew, expression, motif, KLF5
Received: May 06, 2016 Accepted: December 05, 2016 Published: December 10, 2016
Krüppel-like factors (KLFs) are a family of zinc finger transcription factors regulating embryonic development and diseases. The phylogenetics of KLFs has not been studied in tree shrews, an animal lineage with a closer relationship to primates than rodents. Here, we identified 17 KLFs from Chinese tree shrew (Tupaia belangeri chinensis). KLF proteins are highly conserved among humans, monkeys, rats, mice and tree shrews compared to zebrafish and chickens. The CtBP binding site, Sin3A binding site and nuclear localization signals are largely conserved between tree shrews and human beings. Tupaia belangeri (Tb) KLF5 contains several conserved post-transcriptional modification motifs. Moreover, the mRNA and protein expression patterns of multiple tbKLFs are tissue-specific . TbKLF5, like hKLF5, significantly promotes NIH3T3 cell proliferation in vitro. These results provide insight for future studies regarding the structure and function of the tbKLF gene family.
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