Oncotarget

Research Papers: Immunology:

Interleukin-8 holds promise to serve as a molecular adjuvant in DNA vaccination model against Streptococcus iniae infection in fish

Erlong Wang, Bo Long, Kaiyu Wang _, Jun Wang, Yang He, Xingli Wang, Qian Yang, Tao Liu, Defang Chen, Yi Geng, Xiaoli Huang, Ping Ouyang and Weimin Lai

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Oncotarget. 2016; 7:83938-83950. https://doi.org/10.18632/oncotarget.13728

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Abstract

Erlong Wang1,*, Bo Long1,*, Kaiyu Wang1,2, Jun Wang1, Yang He1, Xingli Wang1, Qian Yang1, Tao Liu1, Defang Chen3, Yi Geng1,2, Xiaoli Huang3, Ping Ouyang1 and Weimin Lai1

1 Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China

2 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, China

3 Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China

* These authors have contributed equally to this work

Correspondence to:

Kaiyu Wang, email:

Keywords: aquaculture, bacterial diseases, DNA vaccine, interleukin-8, molecular adjuvant, Immunology and Microbiology Section, Immune response, Immunity

Received: August 20, 2016 Accepted: November 15, 2016 Published: November 30, 2016

Abstract

DNA vaccines had been widely used in animal models against various viral infections, while it was not so convincing for many infectious diseases especially bacterial disease in aquaculture. Interleukin-8(IL-8) as one of the CXC chemokines, its immunological role and adjuvant potential which had been proved in mammals were rarely reported in fish species. In this study, recombination plasmid pcDNA3.1/IL-8(pcIL-8) was conducted and the capacity of IL-8 as molecular adjuvant was explored from several aspects by co-injecting with a DNA vaccine encoding α-enolase(pcENO) against Streptococcus iniae infection in channel catfish. The results suggested that co-injection of pcIL-8 with DNA vaccine increased the innate immunity and specific antibody levels, as well as increased the immune-related genes involving in pro-inflammatory response, humoral and cellular immunity. Moreover, pcIL-8 enhanced the immunoprotection of pcENO with the relative percent survival(RPS) of 60% to 80% against S.iniae infection at 4 week post vaccination(p.v.), with the significantly higher RPS of 73.33% in pcENO+pcIL-8 group compared with that of pcENO alone(53.33%) at challenge test of 8 weeks p.v. Taken together, these results indicate pcIL-8 as a molecular adjuvant co-injected with DNA vaccine not only improves the immunoprotection but also maintains long period of immunity for channel catfish against S.iniae infection. Our study signifies that IL-8 holds promise to serve as a potential adjuvant in DNA vaccines against bacterial infections for long time.


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