Oncotarget

Research Papers:

siRNA-mediated suppression of collagen type iv alpha 2 (COL4A2) mRNA inhibits triple-negative breast cancer cell proliferation and migration

He Jing Song, Guan Hong, Jianbo Yang, Zheng Duo, Fu Li, Chen Wei Cai, Luo Xue Ying, Mao You Sheng, OuYang Yi Wen, Pan Yue and Chang Zou _

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Oncotarget. 2017; 8:2585-2593. https://doi.org/10.18632/oncotarget.13716

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Abstract

He JingSong1,*, Guan Hong2,*, Jianbo Yang3,*, Zheng Duo4, Fu Li5, Chen WeiCai1, Luo XueYing1, Mao YouSheng1, OuYang YiWen1, Pan Yue1, Chang Zou6

1Department of Breast Surgery, The First Affiliated Hospital of Shenzhen University, Second People’s Hospital of Shen Zhen, Shen Zhen, 518035, China

2Department of Pathology, The First Affiliated Hospital of Shenzhen University, Second People’s Hospital of Shen Zhen, Shen Zhen 518035, China

3Department of Laboratory Medicine and Pathology, Masonic Cancer Center, University of Minnesota, UMN Twin Cities, MN 55455, USA

4Shenzhen Key Laboratory of Translational Medicine of Tumor, Department of Cell Biology and Genetics, School of Medicine, Shenzhen University, Shenzhen, Guangdong, 518060, China

5Department of Pharmacology, Shenzhen Key Laboratory of Translational Medicine of Tumor and Cancer Research Centre, School of Medicine, Shenzhen University, Shenzhen, 518060 China

6Clinical Medical Research Center, Shen Zhen People’s Hospital, The Second Clinical Medical College of Jinan University, 518020, China

*These authors contribute equally to this work

Correspondence to:

He JingSong, email: [email protected]

Chang Zou, email: [email protected]

Keywords: triple-negative breast cancer (TNBC), COL4A2, small interfering RNA (siRNA), lentiviral vector

Received: October 19, 2016     Accepted: November 21, 2016     Published: November 30, 2016

ABSTRACT

Triple-negative breast cancer (TNBC) is more aggressive than other breast cancer subtypes. Collagen type IV alpha 2 (COL4A2), a major component of the basement membrane, dynamically influences a wide range of biological processes, including cancer pathogenesis and progression. This study evaluated the effects of COL4A2 siRNA delivered by lentiviral vector to TNBC cells. COL4A2 siRNA lenti-viral vector was constructed and transfected into MDA-MB-231 and MDA-MB-468 cells. The COL4A2 mRNA levels were quantified by RT-PCR. CCK8 assay was performed to evaluate cell proliferation and migration. Cell migration and invasion assays were carried out using Transwell. Cell apoptosis and cell cycle analyses were conducted using flow cytometric approach. We found that COL4A2 mRNA levels were significantly down-regulated in MDA-MB-231 and MDA-MB-468 cells after transfection with COL4A2 siRNA. Furthermore, cell migration and proliferation were significantly decreased and the cell cycle was arrested. Our results indicated that COL4A2 siRNA significantly suppresses the migration and proliferation of TNBC cells. Inhibition of COL4A2 may be a new target for the prevention and treatment of TNBC.


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