Oncotarget

Research Papers:

Loss of ZG16 is regulated by miR-196a and contributes to stemness and progression of colorectal cancer

Xiaobing Chen _, Peng Du, Junjun She, Liang Cao, Yingchao Li and Hongping Xia

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Oncotarget. 2016; 7:86695-86703. https://doi.org/10.18632/oncotarget.13435

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Abstract

Xiaobing Chen1,*, Peng Du2,*, Junjun She3,*, Liang Cao1,*, Yingchao Li4, Hongping Xia3

1Department of Oncology, Henan Cancer Hospital, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China

2Department of Colorectal Surgery, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China

3Department of General Surgery, The First Affiliated Hospital of Xi’An Jiaotong University, Xi’An 710061, China

4Department of Gastroenterology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China

*These authors have contributed equally to this work

Correspondence to:

Xiaobing Chen, email: [email protected]

Junjun She, email: [email protected]

Hongping Xia, email: [email protected]

Keywords: ZG16, Colorectal cancer, LGR5, stemness, miR-196a

Received: August 15, 2016    Accepted: October 31, 2016    Published: November 17, 2016

ABSTRACT

Colorectal cancer (CRC) is one of the most common malignant tumour and the leading cause of cancer-related mortality worldwide. Clarification of the mechanism that underlies CRC tumorigenesis and progression therefore is urgently needed ffor developing novel therapies. Through analysis of The Cancer Genome Atlas (TCGA) dataset, we identified an interesting gene, ZG16, which is significantly decreased in CRC samples compared to adjacent non-tumor tissues and associated with prognosis of patients. We found that the expression of ZG16 correlated with CRC related genes which were regulated by APC/CTNNB1 pathway. Interestingly, the expression of ZG16 was negatively correlated with CRC stem cell marker, LGR5. Overexpression of ZG16 significantly inhibits growth and sphere formation of stem-like CRC cells. Moreover, we also identified an upstream regulator of ZG16, miR-196a, which was significantly overexpressed in CRC and promotes cell growth and stemness. In conclusion, this study demonstrated that loss of ZG16 is regulated by miR-196a and contributes to stemness and progression of CRC, which may provide a promising therapeutic strategy for advanced CRCs.


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