Research Papers: Immunology:

The association between expression of IFIT1 in podocytes of MRL/lpr mice and the renal pathological changes it causes: An animal study

Weiping Hu, Guodong Niu, Hongbo Li, Hanyuan Gao, Rudian Kang, Xiaoqing Chen _ and Ling Lin

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Oncotarget. 2016; 7:76464-76470. https://doi.org/10.18632/oncotarget.13045

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Weiping Hu1,*, Guodong Niu1,*, Hongbo Li1, Hanyuan Gao1, Rudian Kang1, Xiaoqing Chen1 and Ling Lin1

1 Rheumatism Department, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian Province, China

* These authors have contributed equally to this study

Correspondence to:

Xiaoqing Chen, email:

Ling Lin, email:

Keywords: IFIT1, podocytes, animal study, renal pathological changes, lupus nephritis, Immunology and Microbiology Section, Immune response, Immunity

Received: July 28, 2016 Accepted: October 28, 2016 Published: November 03, 2016


Renal damage is the major cause of SLE associated mortality, and IFIT1expression was elevated in SLE cases in accordance of previous studies. Therefore, we conducted an animal study to identify the role of IFIT1 expression in renal pathological changes.18 female MRL/lpr mice and same number of female BALB/c mice were enrolled in present study. Quantitative analysis of urine protein, Complement C3 and C4, and anti-ds DNA antibody were conducted. HE and PAS staining and TEM analysis were employed to observe the pathological changes in renal tissue. Significant elevation on urine protein and anti-dsDNA and reduction on Complement C3 and C4 were observed in MRL/lpr mice when comparing the controls in same age. Staining and TEM analysis observed several pathological changes in glomerulus among MRL/lpr mice, including cellular enlargement, basement membrane thickening, and increased cellularcasts. The linear regression analysis found the optical density of IFIT1 was inversely associated with F-actin, Nephrin, and Podocin, but not Synatopodin. In summary, IFIT1 expression is associated with podocytes damage, and capable of suppressing some proteins essential to glomerular filtration.

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