Long noncoding RNA BX357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of TGF-β1/p38/HSP27 signaling in renal cell carcinoma
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Yiyang Liu1,*, Jian Qian1,*, Xiao Li1,*, Wei Chen1, Aiming Xu1, Kai Zhao1, Yibo Hua1, Zhengkai Huang1, Jianzhong Zhang1, Chao Liang1, Shifeng Su1, Pu Li1, Pengfei Shao1, Jie Li1, Chao Qin1, Zengjun Wang1
1Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
*These authors have contributed equally to this work
Zengjun Wang, email: [email protected]
Chao Qin, email: [email protected]
Keywords: lncRNA BX357664, long noncoding RNA (LncRNA), epithelial-to-mesenchymal transition, TGF-beta 1/p38/HSP27 signaling, renal cell carcinoma
Received: December 06, 2015 Accepted: September 12, 2016 Published: October 27, 2016
Antisense long noncoding RNAs (lncRNAs) are reported to play a regulating role in carcinogenesis of various human malignancies. However, the function of lncRNAs and their underlying mechanism in renal cell carcinoma (RCC) is still unknown. The aims of this study are to investigate the expression of lncRNA BX357664 in RCC and to explore its function in RCC cell lines. As a result, BX357664 was downregulated in RCC according to previous microarray analysis and qualitative real-time polymerase chain reaction. After the upregulation of BX357664, reduced migration, invasion, and proliferation capabilities in RCC cells were detected. Furthermore, Western blot analysis was conducted to identify the influence of BX357664 on epithelial-to-mesenchymal transition, matrix metalloproteinase 2, matrix metalloproteinase 9, and transforming growth factor-beta 1 (TGF-β1)/p38/HSP27 signaling pathway in RCC. Subsequently, upregulating the protein level of TGF-β1 in the presence of BX357664 could rescue the suppression of the malignant behavior mediated by BX357664, indicating that BX357664 attributed its inhibitory role to the suppression of TGF-β1. Therefore, we investigated a novel lncRNA BX357664, which might exhibit its inhibitory role in RCC metastasis and progression by blocking the TGF-β1/p38/HSP27 pathway.
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