Efficacy of glycogen synthase kinase-3β targeting against osteosarcoma via activation of β-catenin

INTRODUCTION

Osteosarcoma is the most common primary malignant bone tumor encountered during childhood and adolescence [1, 2]. Current treatment for this tumor is systemic chemotherapy using multiple agents in combination with limb-salvage surgery. The 5-year survival rate of patients with localized tumor treated by surgery alone was less than 20% prior to the introduction of chemotherapy, but has since been improved by chemotherapy to 70–90% [3, 4]. However, dose-intensive chemotherapy often leads to adverse effects and drug resistance, with some patients unable to tolerate the standard regimens. Despite more effective chemotherapy for primary tumors, the overall 5-year survival rate for patients with recurrent or metastatic osteosarcoma is just 20–30% and this has remained virtually unchanged for the past three decades [3]. Refractory patients should benefit from novel therapies that have less toxicity and resistance, including targeted therapies [3, 5].

Molecular-targeted therapies have been developed recently for the treatment of many cancer types, including osteosarcoma [3, 5, 6]. These targeted agents selectively affect molecules that are deregulated in tumor cells and are therefore associated with less toxicity to normal cells than chemotherapy involving cytotoxic drugs, such as cisplatinum and doxorubicin, which are currently first-line for osteosarcoma [3]. Some of the molecular-targeted agents evaluated in clinical trials for osteosarcoma are directed against receptor-type tyrosine kinases, intracellular signaling pathways, the bone microenvironment (eg., osteoclasts) and the immune system [5, 6]. Thus far, none have been approved for the treatment of osteosarcoma. The failure of some of these trials may be due to tumor heterogeneity [5] and to insufficient preclinical validation of the respective agents. Therefore, the identification of new molecular therapeutic targets has also been highlighted as a high priority for the treatment of refractory osteosarcoma [7, 8].

One emerging molecular target is glycogen synthase kinase-3β (GSK-3β), a serine/threonine protein kinase that maintains homeostasis in normal cells by regulating several fundamental biological pathways [9, 10]. Its known functions and involvement in various primary pathologies has established GSK-3β as a therapeutic target for type 2 diabetes mellitus, neurodegenerative diseases and inflammation [11]. In contrast to its effects against several proto-oncoproteins (eg., β-catenin, cyclin D1) and mediators of epithelial-mesenchymal transition in untransformed cells [12], we have found that deregulated GSK-3β facilitates the progression of gastrointestinal cancers and glioblastoma. This stems from the observation that GSK-3β promotes cell survival, proliferation and invasion by modulating distinct tumor suppressor pathways, cell immortality pathways and cell-motility machinery, thus rendering cancer cells resistant to chemotherapeutic agents and ionizing radiation [13–17]. Together with other studies, our observations have established GSK-3β as a potential therapeutic target in common adult-onset chronic diseases including cancer [13, 18, 19]. Therapeutic effects of GSK-3β inhibition have been shown in epithelial as well as in hematopoietic, neuronal and musculoskeletal cell malignancies including leukemia [20], glioblastoma [16, 17] and rhabdomyosarcoma [21, 22].

In the present study, we explored the expression, activity and potential pathological role of GSK-3β in osteosarcoma, a rare but devastating disease that predominantly affects children and young adults.

RESULTS

Expression and phosphorylation of GSK-3β

Osteosarcoma and osteoblast cells showed similar basal levels of GSK-3β expression (Figure 1). All osteosarcoma cell lines showed higher levels of pGSK- 3β^Y216 (active form) and lower levels of pGSK- 3β^S9 (inactive form) compared to hFOB1.19 osteoblast cells. These findings were consistent with our previous observations [14–16] and led us to hypothesize that osteosarcoma cells depend on deregulated GSK-3β for their survival and proliferation.

Effects of GSK-3β inhibition on osteosarcoma cells

To address the above hypothesis, we examined the effect of GSK-3β inhibition on osteosarcoma cell survival and proliferation. The viability of all osteosarcoma cells was reduced in a dose- and time-dependent manner following treatment with AR-A014418 or SB-216763 (Figure 2), while a less marked effect of these inhibitors was observed in hFOB1.19 osteoblast cells (Supplementary Figure S2). The half-maximal inhibitory concentration (IC₅₀) values at 96 hours after administration of AR-A014418 were 12.4 μmol/L for HOS; 11.7 μmol/L for 143B; 16.0 μmol/L for MG-63; and 14.6 μmol/L for Saos-2 cells. IC50 values for SB-216763 were 10.5 μmol/L for HOS; 17.1 μmol/L for 143B; 14.3 μmol/L for MG-63; and 21.9 μmol/L for Saos-2 cells. These concentrations are within the pharmacological dose ranges for these inhibitors [23, 24]. The GSK-3β inhibitors significantly decreased the relative number of BrdU-positive proliferating osteosarcoma and osteoblast cells (Figure 3A) and increased the relative number of TUNEL-positive cells undergoing apoptosis in the osteosarcoma cell lines, but not in hFOB1.19 osteoblast cells (Figure 3B). Reduction of the level of GSK-3β expression by RNA interference affected osteosarcoma, but not osteoblast proliferation, apoptosis and survival (Figure 4). Consistent with a previous study [25], these results indicate that both activity and expression of GSK-3β are necessary for osteosarcoma cell survival and proliferation, thereby suggesting a potential tumor-promoting role for this kinase in osteosarcomas.

Changes in subcellular localization and activity of β-catenin following GSK-3β inhibition

Previous studies showed the canonical Wnt/β-catenin pathway was inactivated during the development and progression of bone and soft tissue sarcomas including osteosarcoma [26–28]. In contrast to its oncogenic role in many cancer types [29, 30], this observation suggested a tumor suppressor function for the Wnt/β-catenin pathway in osteosarcoma [26–28]. Therefore, we focused on β-catenin, the downstream effector of the Wnt signaling pathway that is phosphorylated by GSK-3β for ubiquitin-mediated proteasomal degradation [29]. Consistent with the results shown above in Figure 1, β-catenin was phosphorylated at the known GSK-3β phospho-acceptor residues (S33, S37 and/or T41) in osteosarcoma cells. Treatment with GSK- 3β inhibitors suppressed the phosphorylation of β-catenin and increased its expression (Figure 5A). GSK-3β inhibitors also induced the nuclear translocation of β-catenin in osteosarcoma cells, whereas the nuclear localization of β-catenin was constitutively observed in hFOB1.19 osteoblasts regardless of treatment with DMSO or GSK-3β inhibitor (Figure 5B, Supplementary Figure S3). The TOP/FOP flash assay showed a significant increase in T-cell factor-dependent promoter activity in osteosarcoma cells following treatment with GSK-3β inhibitors (Figure 5C), reflecting an increase in the co-transcriptional activity of β-catenin.

The influence of β-catenin expression on the therapeutic effects of GSK-3β inhibition of osteosarcoma cells was examined by RNA interference of β-catenin prior to the treatment of cells with GSK-3β inhibitor. Depletion of β-catenin reduced the effects of AR-A014418 on the proliferation and apoptosis of Saos-2 to a greater extent than the other 3 osteosarcoma cell lines (143B, MG-63 and HOS) (Supplementary Figure S4). This suggests the therapeutic effect of GSK-3β inhibition depends not only on β-catenin induction and its co-transcriptional activity but also on known [13, 18] and/or unknown biological mechanism(s) that remain to be further investigated. Treatment with AR-A014418 attenuated proliferation of hFOB1.19 osteoblast cells under conditions of depleted β-catenin expression (Supplementary Figure S4), demonstrating a different role of β-catenin in normal osteoblasts and osteosarcoma cells.

It is of interest whether GSK-3β inhibition has therapeutic efficacy against osteosarcoma cells where increased β-catenin has been linked to malignancy. To address this issue, we investigated the activity of GSK- 3β inhibitors on survival of human osteosarcoma U2OS cells which were reported to depend on β-catenin signaling for cell proliferation [31]. Similar to other osteosarcoma cell lines (Figure 2), both GSK-3β inhibitors dose- and time-dependently suppressed U2OS cells survival (Supplementary Figure S5A). IC₅₀ values at 96 hrs treatment for AR-A014418 and SB-216763 were 18.0 μmol/L and 33.7 μmol/L, respectively. The effect of GSK-3β inhibition was observed in the U2OS cells after knock-down of β-catenin expression (Supplementary Figure S5B), suggesting that β-catenin signaling-independent mechanism(s) such as alteration in NF-κB activity [25] may underlie the efficacy of GSK-3β inhibition against U2OS osteosarcoma cells.

Efficacy of a GSK-3β inhibitor on AKT activity and NF-κB transcriptional activity

It is well documented that activation of phosphatidylinositol-3 kinase by its corresponding tyrosine kinase receptors results in the recruitment of AKT to the cytoplasmic membrane and its activation. AKT then functions upon multiple downstream effectors including GSK-3β [reviewed in 32]. Since AKT inactivates GSK- 3β by phosphorylation of its S9 residue, we examined whether GSK-3β regulates AKT activity in osteosarcoma and osteoblast cells. Western blotting analysis showed that the levels of AKT phosphorylation at its T308 and S473 residues (pAKT^T308, pAKT^S473), reflecting its activity [32], were higher in 3 of 4 osteosarcoma cell lines with low pGSK-3β^S9 (as shown in Figure 1) than hFOB1.19 osteoblast cells (Supplementary Figure S6A). Treatment with 25 μmol/L AR-A014418 for 72 hrs did not change the levels of pAKT^T308 and pAKT^S473 in osteosarcoma and osteoblast cells. This is consistent with the fact that AKT functions upstream of GSK-3β [32] and with our previous study showing no correlation in the phosphorylation-dependent activities between AKT and GSK-3β in colorectal cancer [33].

Based on the pioneering study showing that GSK- 3β is required for NF-κB activation in mouse embryonic hepatocyte survival [34], previous studies focused on the role of GSK-3β in regulating NF-κB activity in cancer cells [25, 35, 36]. Consistent with these studies on pancreatic cancer and osteosarcoma [25, 35, 36], treatment with 25 μmol/L AR-A014418 for 72 hrs attenuated the relative transcriptional activity of NF-κB in 3 of 4 osteosarcoma cell lines, but not in hFOB1.19 osteoblast cells (Supplementary Figure S6B). Together with the previous study showing that GSK-3β inhibition suppressed NF-κB transcriptional activity in U2OS osteosarcoma cells [25], these results suggest that GSK-3β participates in osteosarcoma cell survival by activation of NF-κB and inactivation of the β-catenin osteosarcoma suppressor, in the context of cancer cell types with different genetic and molecular characteristics.

Effect of GSK-3β inhibitors in an orthotopic mouse model of osteosarcoma

Mice with orthotopic 143B tumors were treated by intraperitoneal injection of DMSO and either AR- A014418 or SB-216763 (2 mg/kg body weight each) three times per week for 5 weeks (Supplementary Figure S1). Administration of the GSK-3β inhibitors significantly reduced the tumor volume in mice as early as 2 weeks after treatment was initiated (Figure 6A, Supplementary Figure S7A). At the end of the study (8 weeks after treatment was started), tumors from GSK-3β inhibitor-treated mice weighed significantly less than those from the control group (Figure 6B, Supplementary Figure S7B). As reported in our previous studies [14, 15, 37, 38], no adverse effects were observed in mice during treatment with DMSO or either of the GSK-3β inhibitors. No significant differences in body weight were found between the groups of mice treated with DMSO, AR-A014418 and/or SB-216763. Gross observation and histological examination of the treated mice at autopsy revealed no pathological findings and no primary or metastatic tumors in the major vital organs including lungs, liver, gastrointestinal tract, pancreas and kidneys (data not shown).

Histological observation showed that orthotopically- transplanted cancer cells proliferated out from the tibia toward the surrounding limb tissue (Figure 6C, Supplementary Figure S8). The tumors in sham (DMSO)-treated mice showed medullary proliferation of tumor cells with plump cytoplasm. Compared to the controls, tumors in mice treated with GSK-3β inhibitors consisted of fibroblast-like cancer cells and were associated with reactive stromal and inflammatory cells. Immunohistochemically, nuclear localization of β-catenin in the cancer cells was frequently observed in tumors treated with GSK-3β inhibitors, whereas weak cytoplasmic expression was found in control tumors (Figure 6C; Supplementary Figure S9). The present experiment is proof-of-principle that GSK-3β inhibitors are active in a mouse models representing clinical osteosarcoma.

DISCUSSION

Although several molecular targets with therapeutic potential have been proposed for osteosarcoma, the trials involving these targets have shown insufficient clinical benefit in refractory osteosarcoma patients [7, 8]. This has led us to address whether GSK-3β could be a clinically-useful target for the treatment of osteosarcoma. The present study found that osteosarcoma cells predominantly express the active form of GSK- 3β (pGSK-3β^Y216) that we and others have shown contributes to cancer-cell survival, proliferation and invasion in several malignancies [14–17, 20–22]. Consistent with this observation, we demonstrate here that inhibition of either the activity or expression of GSK- 3β suppresses cell survival and proliferation and induces apoptosis in osteosarcoma cells. Furthermore, we have confirmed the efficacy of pharmacological GSK- 3β inhibitors against orthotopic tumor growth in mice. A large-scale experiment to further confirm these results will be performed in our future studies.

It is important to confirm the efficacy of the two inhibitors are specific to their effects against GSK-3β activity, and not non-specific or off-target effects. For this, we tested the GSK-3β-specific siRNA on osteosarcoma cells. In many cases, effect of an enzyme largely depends on its biological or catalytic activity rather than on its amount. GSK-3β is also the case and we previously reported that the pharmacological GSK-3β inhibitors (AR-A014418, SB-216763) inactivate GSK-3β in cancer cells within an hour after treatment and that the GSK-3β-specific siRNA takes longer than 48 hours to efficiently, but not completely, deplete GSK-3β expression in the same cells [38]. Therefore the pharmacological inhibitors more quickly inhibit GSK-3β activity in cells and lead to a stronger biological effect on cancer cells than treatment with GSK-3β-specific siRNA. However, both pharmacological inhibition of GSK-3β and depletion of GSK-3β by siRNA inhibited proliferation of osteosarcoma cells, suggesting that the pharmacological doses used to inhibit GSK-3β were on target. Together with an earlier study [25], our results confirm that osteosarcoma cells depend upon active GSK- 3β for their survival and proliferation, thus highlighting the potential benefit of targeting GSK-3β for the treatment of osteosarcoma.

An important novel finding relating to therapy was that GSK-3β inhibition had very little effect on the survival or apoptosis of untransformed hFOB1.19 osteoblasts. This was not reported in the earlier study [25] and is consistent with other studies showing that GSK-3β inhibition does not influence the survival or growth of non-neoplastic cells including human embryonic kidney cells (HEK293) [38] and embryonic lung and dermal fibroblasts (WI38, NIH- 3T3) [39]. Compared to osteosarcoma cells, we observed that hFOB1.19 osteoblast cells expressed a lower level of the active form (pGSK-3β^Y216) and higher level of the inactive form (pGSK-3β^S9) of this kinase. Our finding suggests that GSK-3β is inactive in normal cells, as we reported previously [15, 38, 33], thus rendering them less sensitive to GSK-3β inhibitors. The small effect of GSK-3β inhibitors on hFOB1.19 cells and large effect on mice with orthotopic tumors further support the use of these agents for treatment of osteosarcoma in the clinical setting. Larger scale experiments of the GSK-3β-targeted therapy will be performed in future studies.

An earlier study reported that GSK-3β promotes the survival and proliferation of osteosarcoma cells as well as their insensitivity to conventional chemotherapy [25], based on the known role for GSK-3β in positively regulating NF-κB-mediated signaling [35, 36]. Interestingly, in the current study we showed the therapeutic efficacy of GSK-3β inhibition was associated with increased expression, nuclear translocation and co-transcriptional activity of β-catenin. Although activation of the Wnt/β-catenin pathway has been associated with the development and progression of various cancer types such as colorectal cancer [29, 30], inactivation of this pathway has also been observed in bone and soft tissue sarcomas including osteosarcoma [26–28]. In contrast to an earlier study [25], the present results suggest that stabilization and activation of β-catenin may be a mechanism which underlies the efficacy of GSK-3β inhibition in osteosarcoma. To address this important issue, we need to identify molecular pathway(s) under the control of activated β-catenin following GSK-3β inhibition and this will be carried out in future experiments. Future experiments will also address osteosarcoma cells in which increased β-catenin is linked to malignancy. There is growing evidence the Wnt/β-catenin pathway plays a key role in bone formation and homeostasis by mediating osteoblastogenesis from progenitor cells, osteoblast differentiation, and by interfering with osteoclastogenesis [40–43]. It has been reported that osteoclasts in the tumor microenvironment facilitate the progression of osteosarcoma and hence these cells are considered a therapeutic target for osteosarcoma treatment [5, 6]. Thus, inhibition of GSK-3β in osteosarcoma may potentially confer three therapeutic advantages: activation of the β-catenin-mediated pathway to inhibit tumor progression; attenuation of the activity of tumor-associated osteoclasts; and the enhancement of local bone preservation.

The development and indication of GSK-3β inhibitors for the treatment of chronic diseases such as cancer requires a strong awareness of safety issues. The most serious concern is the possibility that long-term GSK-3β inhibition could initiate carcinogenesis due to the well-known role of this kinase in negatively regulating various proto-oncoproteins, in particular β-catenin [9–12, 29, 30]. To date, however, no studies have shown cellular neoplastic transformation via the activation of proto-oncoproteins, or demonstrated an increased incidence of cancer development or mortality following treatment with lithium, the classical GSK-3β inhibitor prescribed in clinical practice [44]. Primary tumor development was not observed in rodents following treatment with GSK-3β inhibitors in the present study or indeed in any previous studies [14, 15, 37, 38]. In contrast to its therapeutic efficacy against tumor-initiating (cancer stem) cells [45], inhibition of GSK-3β positively regulates the pluripotency of embryonic stem cells and the repopulation of hematopoietic stem cells via activation of the Wnt and hedgehog pathways, respectively [46, 47]. Moreover, GSK-3β inhibition has been shown to maintain the stemness of mesenchymal stem cells [48]. Therefore, inhibition of GSK-3β may provide indirect benefits for the treatment of osteosarcoma by limiting the extent of chemotherapy-associated bone marrow injury and by preserving tumor-adjacent stromal cells.

Despite recent advances in intensive multi-agent chemotherapy, tumor recurrence has been observed in 30–40% of osteosarcoma patients [49]. Tumor resistance to chemotherapeutic agents and a lack of suitable biomarkers for predicting the response to chemotherapeutic agents represent major obstacles for the improved treatment of refractory osteosarcoma patients [3, 5, 6]. A recent study showed that expression levels of GSK-3β, integrin β5 and focal-adhesion kinase in the primary tumors of osteosarcoma patients were significant predictors of poor response to neoadjuvant chemotherapy [50]. We previously reported that GSK-3β inhibition sensitizes pancreatic cancer and glioblastoma cells to gemcitabine and temozolomide, respectively, as well as sensitizing them to ionizing radiation through the modulation of distinct molecular pathways [14–17]. It is therefore important to address in future work whether GSK-3β inhibition can also modulate other chemotherapy drugs in osteosarcoma.

Previously-developed concepts and strategies of highly selective tumor targeting can take advantage of molecular targeting of tumors, including tissue-selective therapy which focuses on unique differences between normal and tumor tissues [51–56].

MATERIALS AND METHODS

Ethics statement

All animal studies were conducted with an AntiCancer Institutional Animal Care and Use Committee (IACUC)-protocol specifically approved for this study and in accordance with the principals and procedures outlined in the National Institute of Health Guide for the Care and Use of Animals under Assurance Number A3873-1. In order to minimize any suffering of the animals, anesthesia and analgesics were used for all surgical experiments. Animals were anesthetized by intramuscular injection of a 0.02 ml solution of 20 mg/kg ketamine, 15.2 mg/kg xylazine, and 0.48 mg/kg acepromazine maleate. The response of animals during surgery was monitored to ensure adequate depth of anesthesia. Ibuprofen (7.5 mg/kg orally in drinking water every 24 hours for 7 days post-surgery) was used in order to provide analgesia post-operatively in the surgically-treated animals. The animals were observed on a daily basis and humanely sacrificed by CO₂ inhalation when they met the following humane endpoint criteria: prostration, skin lesions, significant body weight loss, difficulty in breathing, epistaxis, rotational motion and body temperature drop. The use of animals was necessary to evaluate drugs against osteosarcoma in vivo. Animals were housed with no more than 5 per cage. Animals were housed in a barrier facility on a high efficiency particulate arrestance (HEPA)-filtered rack under standard conditions of 12-hour light/dark cycles. The animals were fed an autoclaved laboratory rodent diet.

Cell lines and culture

Human osteosarcoma (HOS, 143B, MG-63, Saos-2) [57–67] and osteoblast (hFOB1.19) [67] cell lines were obtained from the American Type Culture Collection (Manassas, VA) and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Wako, Osaka, Japan) and DMEM/Ham’s F12 (Wako), respectively, both supplemented with 10% fetal bovine serum (FBS). The human osteosarcoma U2OS cell line is from the Human Cancer Cell Line Bank at the Kanazawa University Cancer Research Institute. The cells were maintained in RPMI-1640 (Sigma-Aldrich, St. Louis, MO) containing 10% FBS. This cell line was reported to depend on β-catenin signaling for its proliferation and invasion [31].

Western blotting

Cellular protein was extracted with cell lysis buffer (CelLytic-MT, Sigma-Aldrich). A 30 μg aliquot of protein extract was analyzed by Western immunoblotting for the proteins of interest as described previously [15, 16]. The amount of protein in each sample was normalized to the expression of β-actin. The following primary antibodies were used at the dilutions shown against the indicated proteins: both GSK-3 isoforms (GSK-3α and β) (1:1,000, Millipore, Billerica, MA); GSK-3β (1:1,000, BD Biosciences, Lexington, KY); GSK-3β fractions phosphorylated at the serine (S) 9 residue (pGSK-3β^S9), (1:1,000, Cell Signaling Technology, Beverly, MA); and at the tyrosine (Y) 216 residue (pGSK-3β^Y216) (1:1,000, BD Biosciences); β-catenin (1:1,000; BD Biosciences); β-catenin phosphorylated at S33, S37 and/or threonine (T) 41 residues (p-β-catenin^S33/37/T41) (Cell Signaling Technology); AKT (1:1,000) and its fractions phosphorylated at T308 residue (pAKT^T308) (1:1,000) and at S473 residue (pAKT^S473) (1:1,000) (Cell Signaling Technology); and β-actin (1:4,000, Ambion, Austin, TX).

Efficacy of GSK-3β inhibitors on cell survival, proliferation, and apoptosis in vitro

Cells seeded in 96-well plates were treated with dimethyl sulfoxide (DMSO) (Sigma-Aldrich) or the GSK-3β-specific inhibitors AR-A014418 (Calbiochem, San Diego, CA) [23] or SB-216763 (Sigma-Aldrich) [24] dissolved in DMSO and medium at the indicated concentrations that are within the reported pharmacological dose ranges [23, 24]. At the designated time points (0, 24, 48, 72, 96 hours) the relative number of viable cells was determined using a WST-8 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate) assay kit (Cell Counting Kit-8, Wako). Cell proliferation was evaluated following treatment with DMSO or with either of the two GSK- 3β inhibitors (25 μmol/L each) for 72 hours by using the BrdU enzyme-linked immunosorbent assay (ELISA) kit (Fluoroskan Ascent FL^®; Labsystems, Thermo Fisher Scientific, Waltham, MA). Apoptosis was measured using a Click-iT terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) Alexa Fluor Imaging Assay kit (Life Technologies, Carlsbad, CA) following treatment with DMSO or with either GSK-3β inhibitor for 12 hours. In the assays for cell survival, proliferation and apoptosis, the values are expressed as the mean ± standard deviation (SD) of the 6-well set for DMSO and each inhibitor.

The specific effects of these small molecule inhibitors against GSK-3β activity are described in previous studies [23, 24]. These studies reported that while the biochemical IC₅₀ values of AR-A014418 and SB-216763 are 104 nmol/l and 34.3 nmol/l, respectively, their pharmacological efficacy, such as on tau protein phosphorylation (AR-A014418) and on glucose incorporation into glycogen and activation of glycogen synthase (SB-216763) was observed in the dose range of 1 μmol/l to 50 μmol/l. Importantly, it was reported that 10 μmol/l AR-A014418 does not inhibit the activity of 26 closely related kinases and is therefore considered highly specific for GSK-3β [23]. Given the disparity in doses for biochemical compared to pharmacologic inhibition of GSK-3β described above, caution should be applied in studying these molecules. Since no subsequent information has been available for the effect of AR-A014418 on activity of kinases other than those reported previously [23], the effects of GSK-3β RNA interference were examined on cells transfected with either non-specific or GSK-3β-specific small interfering (si)RNA as described below.

RNA interference

To examine the efficacy of GSK-3β expression on cell survival, proliferation and apoptosis, we used siRNA specific to human GSK-3β (target sequence, 5′- GCUCCAGAUCAUGAGAAAGCUAGAU-3′; GSK- 3β Validated Stealth RNAi) and negative control siRNA (Stealth RNAi Negative Control Low GC duplex), both purchased from Invitrogen, Life Technologies. The specificity of GSK-3β-specific siRNA was confirmed in our previous studies [15, 16]. The effect of siRNA on GSK-3β expression was observed by Western blotting with an antibody that recognizes both GSK-3α and GSK-3β (Millipore) as described above. Cells were seeded in 96- well plates and transfected with 20 nmol/l of either GSK-3β-specific or negative control siRNA using Lipofectamine RNAiMAX (Invitrogen). At 96 hours after transfection of siRNA, the relative number of viable cells and the extent of apoptosis were determined as described above.

The efficacy of GSK-3β inhibitors on osteosarcoma cell proliferation and apoptosis was examined under conditions of depleted β-catenin. Osteosarcoma cells were transfected with 15 nmol/l non-specific (SignalSilence control siRNA, Cell Signaling Technology) or β-catenin-specific siRNA (SignalSilence β-catenin siRNA II, Cell Signaling Technology) for 18 hrs. The cells were then seeded in a 96-well plate and examined for the efficacy of 25 μmol/l AR-A014418 on cell proliferation and apoptosis as described above. Prior to this, the efficiency of β-catenin knockdown in the cells was confirmed by Western blotting with antibody to β-catenin (BD Biosciences) as described above.

Immunofluorescence and fluorescence staining

Osteosarcoma and osteoblast cells, grown on cover slips, were treated with either DMSO or 25 μmol/l AR-A014418 for 24 hrs and then fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton-X (Sigma-Aldrich). The fixed and permeabilized cells were incubated with mouse monoclonal antibody to β-catenin (BD Bioscience; diluted 1:200) at 4°C overnight and then with Alexa Flour^® 488-labeled anti-mouse IgG (Invitrogen; diluted 1:1,000) at room temperature for 40 min. After washing off excess antibody, cell nuclei were counterstained with Hoechst 33342 (Molecular Probes) for 20 min at room temperature. The cells were observed by fluorescence microscopy (Keyence) for the expression and subcellular localization of β-catenin. The effect of GSK-3β inhibition on the subcellular localization of β-catenin was quantified in cells treated with DMSO or with AR-A014418. In each assay, the mean percentage of cells that was positive for nuclear β-catenin localization was calculated with SDs in 3 separate microscopic fields.

TOP/FOP flash assay

To examine changes in the co-transcriptional activity of β-catenin following treatment with the GSK-3β inhibitors, we employed the TOP/FOP reporter system using the dual luciferase kit (Dual-GloTM Luciferase Assay System, Promega, Madison, WI) according to the manufacturer’s instruction. The vectors used by this system were M50 Super 8X TOPFlash and M50 Super 8X FOPFlash (Addgene, Cambridge, MA). Cells were cultured for 14 hours and then transfected with both vectors using the Lipofectamine^® 2000 transfection reagent (Life Technologies). At 15 hours after transfection, cells were treated with DMSO, 25 μmol/l AR-A014418 or SB-216763 for 12 hours. Luciferase activity in the cells was then measured using a Centro LB 960 Microplate Luminometer (Berthold Technologies, Germany) at 12 hours after treatment with DMSO or with one of the two GSK-3β inhibitors. The relative value for the co-transcriptional activity of β-catenin in the cells is expressed as the mean ± SD of four separate experiments for the respective conditions performed in duplicate.

Nuclear factor (NF)-κB transcriptional activity assay

Changes in NF-κB transcriptional activity following GSK-3β inhibition were measured for osteosarcoma and osteoblast cell lines with a luciferase reporter assay in the presence of DMSO or 25 μmol/L AR-A014418, according to our previous study [16]. This assay used the pGL4.32[luc2P/NF-κB-RE/Hygro] vector, pRL Renilla luciferase control vector (pRL-SV40 Vector) and the Dual-Luciferase^® Reporter Assay System (Promega). In each assay, the luciferase activity of pGL4.32[luc2P/NF-κB -RE/Hygro] vector-transfected cells was normalized to the co-transfected pRL-SV40 vector. The relative NF-κB transcriptional activity was then compared between the same cells treated with DMSO and 25 μmol/l AR-A014418 for 72 hrs.

Orthotopic mouse model of osteosarcoma and treatment

The efficacy of GSK-3β inhibition on tumor growth was examined on 143B cells [58–65] transplanted orthotopically in athymic nude mice (AntiCancer, San Diego, CA). Twelve mice, six to eight weeks old, were anesthetized by a ketamine mixture (10 μl ketamine HCl, 7.6 μl xylazine, 2.4 μl acepromazine maleate, and 10 μl of sterilized water) via subcutaneous injection. The left leg was sterilized with alcohol and a 5 mm midline skin incision was made below the knee joint to expose the tibial tuberosity. 143B cells (5 × 10⁵ cells/5 μl) were suspended in Matrigel (BD Bioscience) and injected into the medullary cavity of the left tibia with a 0.5 ml 28 G latex-free insulin syringe (TYCO Health Group LP, Mansfield, MA). The skin was closed with a 6–0 suture as described previously [65]. These mice were assigned to 3 groups (4 mice each) and given intraperitoneal injection of 75% DMSO or of either GSK-3β inhibitor at a dose of 2 mg/kg body weight for three times per week and for 5 weeks (Supplementary Figure S1) as described in our previous studies [37, 38]. Assuming that 60% of body weight is accounted by body fluid, a dose of 2 mg/kg body weight corresponds approximately to a concentration of 10 μmol/l in culture medium for both inhibitors [37]. Throughout the experiment, all mice were carefully observed daily for any adverse events and the tumors were measured for size in two dimensions every week. Tumor volume (cm³) was calculated using the formula: 0.5 × a² × b, where a is the smallest tumor diameter (cm) and b is the largest [68].

All mice were euthanized after the completion of treatment. At necropsy, tumor and vital organs were removed and fixed in 10% neutral-buffered formalin. Tumor weight (g) was measured before fixation. Tumor tissues were decalcified after fixation and embedded in paraffin for histopathologic and immunohistochemical examination. Paraffin sections of the respective tumors were stained with hematoxylin and eosin (H&E) and immunostained with rabbit monoclonal antibody to β-catenin (diluted 1:100; Cell Signaling Technology) according to our previous studies [37, 38].

All animal experiments were undertaken in accordance with the Guidelines for the Care and Use of Laboratory Animals under National Institutes of Health assurance number A3873-01 and the U.S. Public Health Service Policy on Humane Care and Use of Laboratory Animals [69] that also covers the national guidelines in Japan [70].

Statistical analysis

Data were evaluated statistically using the Student’s t test (cell survival, proliferation and apoptosis) and Mann-Whitney U test (TOP/FOP Flash assay, volume of orthograft tumors); with a p value of < 0.05 considered statistically significant.

Abbreviations

BrdU, 5-bromo-2′-deoxyuridine; DMSO, dimethyl sulfoxide; ELISA, enzyme-linked immunosorbent assay; FBS, fetal bovine serum; GSK-3β, glycogen synthase kinase-3β; NF-κB, nuclear factor-κB; SD(s), standard deviation(s); siRNA, short interfering RNA; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling.

ACKNOWLEDGMENTS AND FUNDING

We thank Dr. Hiroko Oshima and Dr. Masanobu Oshima (Cancer Research Institute, Kanazawa University) for assistance in the TOP/FOP flash assay; Dr. Yoshio Endo (Cancer Research Institute, Kanazawa University) for providing U2OS osteosarcoma cell line; and Dr. Barry Iacopetta (University of Western Australia) for critical review of the manuscript.

CONFLICTS OF INTEREST

The authors have no conflicts of interest.

REFERENCES

1. Ottaviani G, Jaffe N. The epidemiology of osteosarcoma. Cancer Treat Res. 2009; 52:3–13.

2. Whelan J, McTiernan A, Cooper N, Wong YK, Francis M, Vernon S, Strauss SJ. Incidence and survival of malignant bone sarcomas in England 1979–2007. Int J Cancer. 2012; 131:E508–E517.

3. Luetke A, Meyers PA, Lewis I, Juergens H. Osteosarcoma treatment – where do we stand? A state of the art review. Cancer Treat Rev. 2014; 40:523–532.

4. Tsuchiya H, Tomita K, Mori Y, Asada N, Morinaga T, Kitano S, Yamamoto N. Caffeine-assisted chemotherapy and minimized tumor excision for nonmetastatic osteosarcoma. Anticancer Res. 1998; 18:657–666.

5. Botter SM, Neri D, Fuchs B. Recent advances in osteosarcoma. Curr Opin Pharmacol. 2014; 16:15–23.

6. Kansara M, Teng MW, Smyth MJ, Thomas DM. Translational biology of osteosarcoma. Nat Rev Cancer. 2014; 14:722–735.

7. Yang J, Zhang W. New molecular insights into osteosarcoma targeted therapy. Curr Opin Oncol. 2013; 25:398–406.

8. Khanna C, Fan TM, Gorlick R, Helman LJ, Kleinerman ES, Adamson PC, Houghton PJ, Tap WD, Welch DR, Steeg PS, Merlino G, Sorensen PH, Meltzer P, et al. Toward a drug development path that targets metastatic progression in osteosarcoma. Clin Cancer Res. 2014; 20:4200–4209.

9. Doble BW, Woodgett JR. GSK-3: tricks of the trade for a multi-tasking kinase. J Cell Sci. 2003; 116:1175–1186.

10. Jope RS, Johnson GV. The glamour and gloom of glybogen synthase kinase-3. Trends Biochem Sci. 2004; 29:95–102.

11. Jope RS, Yuskaitis CJ, Beurel E. Glycogen synthase kinase-3 (GSK-3): inflammation, disease, and therapeutics. Neurochem Res. 2007; 32:577–595.

12. Luo J. Glycogen synthase kinase 3β (GSK3β) in tumorigenesis and cancer chemotherapy. Cancer Lett. 2009; 273:194–200.

13. Miyashita K, Nakada M, Shakoori A, Ishigaki Y, Shimasaki T, Motoo Y, Kawakami K, Minamoto T. An emerging strategy for cancer treatment targeting aberrant glycogen synthase kinase 3β. Anticance. Agents Med Chem. 2009; 9:1114–1122.

14. Shimasaki T, Ishigaki Y, Nakamura Y, Takata T, Nakaya N, Nakajima H, Sato I, Zhao X, Kitano A, Kawakami K, Tanaka T, Takegami T, Tomosugi N, et al. Glycogen synthase kinase 3β inhibition sensitizes pancreatic cancer cells to gemcitabine. J Gastroenterol. 2012; 47:321–333.

15. Kitano A, Shimasaki T, Chikano Y, Nakada M, Hirose M, Higashi T, Ishigaki Y, Endo Y, Takino T, Sato H, Sai Y, Miyamoto K, Motoo Y, et al. Aberrant glycogen synthase kinase 3β is involved in pancreatic cancer cell invasion and resistance to therapy. PLoS ONE. 2013; 8:e55289.

16. Miyashita K, Kawakami K, Nakada M, Mai W, Shakoori A, Fujisawa H, Hayashi Y, Hamada J, Minamoto T. Potential therapeutic effect of glycogen synthase kinase 3β inhibition against human glioblastoma. Clin Cancer Res. 2009; 15:887–897.

17. Pyko IV, Nakada M, Sabit H, Teng L, Furuyama N, Hayashi Y, Kawakami K, Minamoto T, Fedulau AS, Hamada J. Glycogen synthase kinase 3β inhibition sensitizes human glioblastoma cells to temozolomide by affecting O6-methylguanine DNA methyltransferase promoter methylation via c-Myc signaling. Carcinogenesis. 2013; 34:2206–2217.

18. McCubrey JA, Steelman LS, Bertrand FE, Davis NM, Sokolosky M, Abrams SL, Montalto G, D’Assoro AB, Libra M, Nicoletti F, Maestro R, Basecke J, Rakus D, et al. GSK-3 as potential target for therapeutic intervention in cancer. Oncotarget. 2014; 5:2881–2911. doi: 10.18632/oncotarget.2037.

19. Phukan S, Babu VS, Kannoji A, Hariharan R, Balaji VN. GSK3β: role in therapeutic landscape and development of modulators. Br J Pharmacol. 2010; 160:1–19.

20. Holmes T, O’Brien TA, Knight R, Lindeman R, Symonds G, Dolnikov A. The role of glycogen synthase kinase-3β in normal haematopoiesis, angiogenesis and leukaemia. Curr Med Chem. 2008; 15:1493–1499.

21. Zeng FY, Dong H, Cui J, Liu L, Chen T. Glycogen synthase kinase 3 regulates PAX3- FKHR-mediated cell proliferation in human alveolar rhabdomyosarcoma cells. Biochem Biophys Res Commun. 2010; 391:1049–1055.

22. Chen EY, DeRan MT, Ignatius MS, Grandinetti KB, Clagg R, McCarthy KM, Lobbardi RM, Brockmann J, Keller C, Wu X, Langenau DM. Glycogen synthase kinase 3 inhibitors induce the canonical WNT/β-catenin pathway to suppress growth and self-renewal in embryonal rhabdomyosarcoma. Proc Natl Acad Sci USA. 2014; 111:5349–5354.

23. Bhat R, Xue Y, Berg S, Hellberg S, Ormo M, Nilsson Y, Radesater AC, Jerning E, Markgren PO, Borgegard T, Nylof M, Gimenez-Cassina A, Hernandez F, et al. Structural insights and biological effects of glycogen synthase kinase 3-specific inhibitor AR-A014418. J Biol Chem. 2003; 278:45937–45945.

24. Coghlan MP, Culbert AA, Cross DA, Corcoran SL, Yates JW, Pearce NJ, Rausch OL, Murphy GJ, Carter PS, Roxbee Cox L, Mills D, Brown MJ, Haigh D, et al. Selective small molecule inhibitors of glycogen synthase kinase-3 modulate glycogen metabolism and gene transcription. Chem Biol. 2000; 7:793–803.

25. Tang QL, Xie XB, Wang J, Chen Q, Han AJ, Zou CY, Yin JQ, Liu DW, Liang Y, Zhao ZQ, Yong BC, Zhang RH, Feng QS, et al. Glycogen synthase kinase-3β, NF-κB signaling, and tumorigenesis of human osteosarcoma. J Natl Cancer Inst. 2012; 104:749–763.

26. Matushansky I, Hernando E, Socci ND, Mills JE, Matos TA, Edgar MA, Singer S, Maki RG, Cordon-Cardo C. Derivation of sarcomas from mesenchymal stem cells via inactivation of the Wnt pathway. J Clin Invest. 2007; 117:3248–3257.

27. Cai Y, Monseny AB, Karperien M, Hogendoorn PC, Zhou G, Cleton-Jansen AM. Inactive Wnt/β-catenin pathway in conventional high-grade osteosarcoma. J Pathol. 2010; 220:24–33.

28. Thomas DM. Wnts, bone and cancer. J Pathol. 2010; 220:1–4.

29. Clevers H, Nusse R. Wnt/β-catenin signaling and diseases. Cell. 2012; 149:1192–1205.

30. Anastas JN, Moon RT. WNT signalling pathways as therapeutic targets in cancer. Nat Rev Cancer. 2013; 13:11–26.

31. Ma Y, Zhu B, Liu X, Yu H, Yong L, Liu X, Shao J, Liu Z. Inhibition of oleandrin on the proliferation show and invasion of osteosarcoma cells in vitro by suppressing Wnt/ β-catenin signaling pathway. J Exp Clin Cancer Res. 2015; 34:115.

32. Faes S, Dormond O. PI3K and AKT: unfaithful partners in cancer. Int J Mol Sci. 2015; 16:21138–21152.

33. Shakoori A, Ougolkov A, Yu ZW, Zhang B, Modarressi MH, Billadeau DD, Mai M, Takahashi Y, Minamoto T. Deregulated GSK3β activity in colorectal cancer: its association with tumor cell survival and proliferation. Biochem Biophys Res Commun. 2005; 334:1365–1373.

34. Hoeflich KP, Luo J, Rubie EA, Tsao MS, Jin O, Woodgett JR. Requirement for glycogen synthase kinase- 3β in cell survival and NF-κB activation. Nature. 2000; 406:86–90.

35. Ougolkov AV, Fernandez-Zapico ME, Savoy DN, Urrutia RA, Billadeau DD. Glycogen synthase kinase- 3β participates in nuclear factor κB-mediated gene transcription and cell survival in pancreatic cancer cells. Cancer Res. 2005; 65:2076–2081.

36. Mamaghani S, Patel S, Hedley DW. Glycogen synthase kinase-3 inhibition disrupts nuclear factor-kappaB activity in pancreatic cancer, but fails to sensitize to gemcitabine chemotherapy. BMC Cancer. 2009; 9:132.

37. Shakoori A, Mai W, Miyashita K, Yasumoto K, Takahashi Y, Ooi A, Kawakami K, Minamoto T. Inhibition of GSK-3β activity attenuates proliferation of human colon cancer cells in rodents. Cancer Sci. 2007; 98:1388–1393.

38. Mai W, Kawakami K, Shakoori A, Kyo S, Miyashita K, Yokoi K, Jin M, Shimasaki T, Motoo Y, Minamoto T. Deregulated GSK3β sustains gastrointestinal cancer cells survival by modulating human telomerase reverse transcriptase and telomerase. Clin Cancer Res. 2009; 15:6810–6819.

39. Kunnimalaiyaan M, Vaccaro AM, Ndiaye MA, Chen H. Inactivation of glycogen synthase kinase-3β, a downstream target of the raf-1 pathway, is associated with growth suppression in medullary thyroid cancer cells. Mol Cancer Ther. 2007; 6:1151–1158.

40. Hartmann C. A Wnt canon orchestrating osteoblastogenesis. Trends Cell Biol. 2006; 16:151–158.

41. Krishnan V, Bryant HU, Macdougald OA. Regulation of bone mass by Wnt signaling. J Clin Invest. 2006; 116:1202–1209.

42. Bodine PV, Komm BS. Wnt signaling and osteoblastogenesis. Rev Endocr Metab Disord. 2006; 7:33–39.

43. Ralston SH, de Crombrugghe B. Genetic regulation of bone mass and susceptibility to osteoporosis. Genes Dev. 2006; 20:2492–2506.

44. Ryves WJ, Dajani R, Pearl L, Harwood AJ. Glycogen synthase kinase-3 inhibition by lithium and beryllium suggests the presence of two magnesium binding sites. Biochem Biophys Res Commun. 2002; 290:967–972.

45. Korur S, Huber RM, Sivasankaran B, Petrich M, Morin P Jr, Hemmings BA, Merlo A, Lino MM. GSK3β regulates differentiation and growth arrest in glioblastoma. PLoS ONE. 2009; 4:e7443.

46. Sato N, Meijer L, Skaltsounis L, Greengard P, Brivanlou AH. Maintenance of pluripotency in human and mouse embryonic stem cells though activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med. 2004; 10:55–63.

47. Trowbridge JJ, Xenocostas A, Moon RT, Bhatia M. Glycogen synthase kinase-3 is an in vivo regulator of hematopoietic stem cell repopulation. Nat Med. 2006; 12:89–98.

48. Cao H, Chu Y, Lv X, Qui P, Liu C, Zhang H, Li D, Peng S, Dou Z, Hua J. GSK3 inhibitor-BIO regulates proliferation of immortalized pancreatic mesenchymal stem cells (iPMSCs). PLoS ONE. 2012; 7:e31502.

49. Pakos EE, Nearchou AD, Grimer RJ, Koumoullis HD, Abudu A, Bramer JA, Jeys LM, Franchi A, Scoccianti G, Campanacci D, Capanna R, Aparicio J, Tabone MD, et al. Prognostic factors and outcomes for osteosarcoma: an international collaboration. Eur J Cancer. 2009; 45:2367–2375.

50. Le GS, Moyal EC, Filleron T, Delisle MB, Chevreau C, Rubie H, Castex MP, de Gauzy JS, Bonnevialle P, Gomez-Brauchet A. The β5/focal adhesion kinase/glycogen synthase kinase 3β integrin pathway in high-grade osteosarcoma: a protein expression profile predictive of response to neoadjuvant chemotherapy. Hum Pathol. 2013; 44:2149–2158.

51. Blagosklonny MV. Matching targets for selective cancer therapy. Drug Discov Today. 2003; 8:1104–1107.

52. Blagosklonny MV. Teratogens as anti-cancer drugs. Cell Cycle. 2005; 4:1518–1521.

53. Blagosklonny MV. Treatment with inhibitors of caspases, that are substrates of drug transporters, selectively permits chemotherapy-induced apoptosis in multidrug-resistant cells but protects normal cells. Leukemia. 2001; 15:936–941.

54. Blagosklonny MV. Target for cancer therapy: proliferating cells or stem cells. Leukemia. 2006; 20:385–391.

55. Apontes P, Leontieva OV, Demidenko ZN, Li F, Blagosklonny MV. Exploring long-term protection of normal human fibroblasts and epithelial cells from chemotherapy in cell culture. Oncotarget. 2011; 2:222–233. doi: 10.18632/oncotarget.248.

56. Blagosklonny MV. Tissue-selective therapy of cancer. Br J Cancer. 2003; 89:1147–1151.

57. Štarha P, Dvořák Z, Trávníček Z. Highly and broad-spectrum in vitro antitumor active cis-Dichloridoplatinum(II) complexes with 7-Azaindoles. PLoS ONE. 2015; 10: e0136338.

58. Hayashi K, Zhao M, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Kishimoto H, Bouvet M, Hoffman RM. Systemic targeting of primary bone tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhymurium. Cell Cycle. 2009; 8:870–875.

59. Kimura H, Tome Y, Momiyama M, Hayashi K, Tsuchiya H, Bouvet M, Hoffman RM. Imaging the inhibition by anti-β1 integrin antibody of lung seeding of single osteosarcoma cells in live mice. Int J Cancer. 2012; 131:2027–2033.

60. Uehara F, Tome Y, Miwa S, Hiroshima Y, Yano S, Yamamoto M, Mii S, Maehara H, Bouvet M, Kanaya F, Hoffman RM. Osteosarcoma cells enhance angiogenesis visualized by color-coded imaging in the in vivo Gelfoam^® assay. J Cell Biochem. 2014; 115:1490–1494.

61. Uehara F, Tome Y, Yano S, Miwa S, Mii S, Hiroshima Y, Bouvet M, Maehara H, Kanaya F, Hoffman RM. A color-coded imaging model of the interaction of αv integrin-GFP expressed in osteosarcoma cells and RFP expressing blood vessels in Gelfoam^® vascularized in vivo. Anticancer Res. 2013; 33:1361–1366.

62. Tome Y, Sugimoto N, Yano S, Momiyama M, Mii S, Maehara H, Bouvet M, Tsuchiya H, Kanaya F, Hoffman RM. Real-time imaging of αv integrin molecular dynamics in osteosarcoma cells in vitro and in vivo. Anticancer Res. 2013; 33:3021–3025.

63. Tome Y, Kimura H, Maehara H, Sugimoto N, Bouvet M, Tsuchiya H, Kanaya F, Hoffman RM. High lung-metastatic variant of human osteosarcoma cells, selected by passage of lung metastasis in nude mice, is associated with increased expression of αvβ3 integrin. Anticancer Res. 2013; 33:3623–3627.

64. Uehara F, Tome Y, Miwa S, Hiroshima Y, Yano S, Yamamoto M, Mii S, Maehara H, Bouvet M, Kanaya F, Hoffman RM. Osteosarcoma cells enhance angiogenesis visualized by color-coded imaging in the in vivo Gelfoam^® assay. J Cell Biochem. 2014; 115:1490–1494.

65. Miwa S, Hiroshima Y, Yano S, Zhang Y, Matsumoto Y, Uehara F, Yamamoto M, Kimura H, Hayashi K, Bouvet M, Tsuchiya H, Hoffman RM. Fluorescence-guided surgery improves outcome in an orthotopic osteosarcoma nude-mouse model. J Orthopaedic Res. 2014; 32:1596–1601.

66. Savu D, Petcu I, Temelie M, Mustaciosu C, Moisoi N. Compartmental stress responses correlate with cell survival in bystander effects induced by the DNA damage agent, bleomycin. Mutat Res. 2015; 771:13–20.

67. Li X, Chen L, Wang W, Meng FB, Zhao RT, Chen Y. (2015) MicroRNA-150 inhibits cell invasion and migration and is downregulated in human osteosarcoma. Cytogenet Genome Res. 2015; 146:124-135.

68. Sun FX, Tohgo A, Bouvet M, Yagi S, Nassirpour R, Moossa AR, Hoffman RM. Efficacy of camptothecin analog DX-8951f (Exatecan Mesylate) on human pancreatic cancer in an orthotopic metastatic model. Cancer Res. 2003; 63:80–85.

69. The Office of Laboratory Animal Welfare, National Institutes of Health, Department of Health and Human Services [Internet]. Guide for the Care and Use of Laboratory Animals, 8th Edition [updated 2008 November 14; cited 2014 December 1]. Available from: http://grants.nih.gov/grants/olaw/Guide-for-the-Care-and-Use-of-Laboratory-Animals.pdf.

70. MEXT LifeScience Portalsite [Internet]. Tokyo, Japan: Fundamental Guidelines for Proper Conduct of Animal Experiment and Related Activities in Academic Research Institutions under the jurisdiction of the Ministry of Education, Culture, Sports, Science and Technology in Japan [updated 2006 June 1; cited 2014 December 1]. Available from: http://www.lifescience.mext.go.jp/policies/pdf/an_material011.pdf.