Research Papers: Pathology:

Wogonin prevents TLR4-NF-κB-medicated neuro-inflammation and improves retinal ganglion cells survival in retina after optic nerve crush

Yue Xu, Boyu Yang, Yaguang Hu, Lin Lu, Xi Lu, Jiawei Wang, Fan Xu, Shanshan Yu, Jingjing Huang _ and Xiaoling Liang

PDF  |  HTML  |  How to cite

Oncotarget. 2016; 7:72503-72517. https://doi.org/10.18632/oncotarget.12700

Metrics: PDF 2089 views  |   HTML 2517 views  |   ?  


Yue Xu1,*, Boyu Yang1,*, Yaguang Hu1,*, Lin Lu1, Xi Lu1, Jiawei Wang1, Fan Xu1, Shanshan Yu1, Jingjing Huang1 and Xiaoling Liang1

1 State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, People’s Republic of China

* These authors have contributed equally to this work

Correspondence to:

Jingjing Huang, email:

Xiaoling Liang, email:

Keywords: wogonin; glaucoma; TLR4-NF-κB pathways; retinal ganglion cells; inflammatory responses; Pathology Section

Received: August 24, 2016 Accepted: October 05, 2016 Published: October 16, 2016


Chronic neuro-inflammation is involved in the death of retinal ganglion cells (RGCs) in glaucoma. The aim of this study is to determine whether wogonin can suppress inflammatory responses and rescue RGCs death after optic nerve crush (ONC), an ideal animal model of glaucoma. Wogonin was administered intraperitoneally 10 min after establishment of ONC model. In this study, wogonin treatment reduced RGCs loss and inhibited RGCs apoptosis demonstrated by the increased Brn3a labeling RGCs at day 14 and the decreased cleaved caspase-3 expression at day 7 after ONC, respectively. In ONC model, number of GFAP-positive glial cells and iba1-positive microglial cells were increased, combined of the elevated level of pro-inflammatory cytokines released in retina at day 7. However, most of these responses were inhibited after wogonin treatment. The level of TLR4 expression, NF-κB-P65 nucleus location and NF-κB-P65 phosphorylation were increased in retina at day 1 after ONC, which was significantly reduced after wogonin treatment. These results demonstrated that wogonin protected RGCs survival and suppressed neuro-inflammation in retina after ONC by inhibiting TLR4-NF-κB pathways. We conclude that wogonin could be a possible strategy for the treatment of glaucoma.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 12700