Oncotarget

Research Papers: Immunology:

Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types

Qingjing Wang, Zhencui Li, Jingxia Lin, Xiuna Wang, Xianbo Deng and Youjun Feng _

PDF  |  HTML  |  How to cite  |  Order a Reprint

Oncotarget. 2016; 7:82112-82122. https://doi.org/10.18632/oncotarget.12621

Metrics: PDF 1363 views  |   HTML 2011 views  |   ?  


Abstract

Qingjing Wang1,*, Zhencui Li1,*, Jingxia Lin1, Xiuna Wang1,3, Xianbo Deng2 and Youjun Feng1

1 Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China

2 College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong, China

3 Fujian Agriculture and Forestry University, Fuzhou, Fujian, China

* The authors have contributed equally to this work

Correspondence to:

Youjun Feng, email:

Xianbo Deng, email:

Keywords: MCR-1, diversity, sequence type (ST), Immunology and Microbiology Section, Immune response, Immunity

Received: August 23, 2016 Accepted: October 07, 2016 Published: October 12, 2016

Abstract

The emergence of the mobilized colistin resistance gene, representing a novel mechanism for bacterial drug resistance, challenges the last resort against the severe infections by Gram-negative bacteria with multi-drug resistances. Very recently, we showed the diversity in the mcr-1-carrying plasmid reservoirs from the gut microbiota. Here, we reported that a similar but more complex scenario is present in the healthy swine populations, Southern China, 2016. Amongst the 1026 pieces of Escherichia coli isolates from 3 different pig farms, 302 E. coli isolates were determined to be positive for the mcr-1 gene (30%, 302/1026). Multi-locus sequence typing assigned no less than 11 kinds of sequence types including one novel Sequence Type to these mcr-1-positive strains. PCR analyses combined with the direct DNA sequencing revealed unexpected complexity of the mcr-1-harbouring plasmids whose backbones are at least grouped into 6 types four of which are new. Transcriptional analyses showed that the mcr-1 promoter of different origins exhibits similar activity. It seems likely that complex dissemination of the diversified mcr-1-bearing plasmids occurs amongst the various ST E. coli inhabiting the healthy swine populations, in Southern China.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 12621