miR-208a-3p suppresses cell apoptosis by targeting PDCD4 in gastric cancer
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Kai Yin1,2,3,*, Minghui Liu2,*, Mei Zhang4,*, Feng Wang1,*, Min Fen1, Zhijian Liu5, Yutao Yuan5, Shanting Gao5, Liuqing Yang5, Weijie Zhang1, Jianguo Zhang6, Baoliang Guo6, Jianwei Xu3, Hongwei Liang2, Xi Chen2, Wenxian Guan1
1Department of Gastrointestinal Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, Jiangsu 210008, China
2State Key Laboratory of Pharmaceutical Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210046, China
3Department of General Surgery, Taixing Hospital Affiliated to Bengbu Medical School, Taixing, Jiangsu 225400, China
4Department of Anesthesiology, Taixing Hospital Affiliated to Bengbu Medical School, Taixing, Jiangsu 225400, China
5Department of Gastrointestinal Surgery, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210008, China
6Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, China
*These authors contributed equally to this work
Wenxian Guan, email: [email protected]
Xi Chen, email: [email protected]
Hongwei Liang, email: [email protected]
Jianwei Xu, email: [email protected]
Keywords: gastric cancer, microRNA, miR-208a-3p, PDCD4, apoptosis
Received: April 24, 2016 Accepted: September 02, 2016 Published: September 13, 2016
Programmed cell death 4 (PDCD4) is a novel tumor suppressor gene and a promising target for anticancer therapies. PDCD4 is frequently downregulated in various human cancers; however, the molecular mechanism accounting for the loss expression of PDCD4 in cancers is not fully understood. In this study, we identified specific targeting sites for miR-208a-3p in the 3’-untranslated region (3′-UTR) of the PDCD4 gene which regulated PDCD4 expression. We demonstrated that miR-208a-3p suppressed apoptosis in gastric cancer cells by targeting PDCD4. We also showed that miR-208a-3p promoted the development of tumor growth in xenograft mice by negatively regulating PDCD4. Taken together, this study revealed a critical role for miR-208a-3p as an oncogenic miRNA in gastric carcinogenesis and it may provide a potential novel target for gastric cancer diagnosis and therapy.
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