Silencing of cZNF292 circular RNA suppresses human glioma tube formation via the Wnt/β-catenin signaling pathway
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Ping Yang1,*, Zhijun Qiu2,*, Yuan Jiang3,*, Lei Dong4, Wensheng Yang5, Chao Gu6, Guang Li6, Yu Zhu1
1Department of Clinical Laboratory, Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vessels and Neural Degeneration, Tianjin, China
2Department of Neurology, Tianjin Medical University General Hospital, Tianjin Neurological Institute, Tianjin, China
3Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, Key Laboratory of Educational Ministry of China, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China
4Department of Pediatrics, Division of Hematology/Oncology, Aflac Cancer and Blood Disorders Center, Emory University School of Medicine, GA, USA
5School of Pharmacal Sciences, Tianjin Medical University, Tianjin, China
6Department of Genetics, College of Basic Medicine, Tianjin Medical University, Tianjin, China
*These authors contributed equally to this work
Yu Zhu, email: [email protected]
Guang Li, email: [email protected]
Ping Yang, email: [email protected]
Chao Gu, email: [email protected]
Keywords: circular RNAs, cZNF292, glioma, tube formation, proliferation
Received: April 30, 2016 Accepted: August 11, 2016 Published: August 23, 2016
CircRNA is a novel type of RNA molecule formed by a covalently closed loop which have no 5’-3’ polarity and possess no polyA tail and relatively stable due to the cyclic structure. Therefore, they may serve as potential targets and diagnosis biomarkers for tumor therapy. cZNF292 is an important circular oncogenic RNA and plays a critical role in the progression of tube formation. This study is aimed at exploring the role of cZNF292 in human glioma tube formation and its potential mechanism of action. We found that cZNF292 silencing suppresses tube formation by inhibiting glioma cell proliferation and cell cycle progression. Cell cycle progression in human glioma U87MG and U251 cells was halted at S/G2/M phase via the Wnt/β-catenin signaling pathway and related genes such as PRR11, Cyclin A, p-CDK2, VEGFR-1/2, p-VEGFR-1/2 and EGFR. The results suggest that cZNF292 silencing plays an important role in the tube formation process and has potential for application as a therapeutic target and biomarker in glioma.
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