Proteomic identification of cyclophilin A as a potential biomarker and therapeutic target in oral submucous fibrosis
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Yao Yuan1,*, Xiaohui Hou2,*, Hui Feng3,*, Rui Liu1,*, Hao Xu1, Wang Gong1, Jing Deng1, Chongkui Sun1, Yijun Gao4, Jieying Peng5, Yingfang Wu5, Jiang Li5, Changyun Fang5, Qianming Chen1
1State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu China, 610041
2Department of Endodontics, School & Hospital of Stomatology, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Tongji University, Shanghai China, 200072
3Xiangya Stomatological Hospital, Central South University, Changsha, China, 410008
4Department of Stomatology, Second Xiangya Hospital, Central South University, Changsha, China, 410008
5Center of Stomatology, Xiangya Hospital, Central South University, Changsha, China, 410008
*These authors have contributed equally to this work
Changyun Fang, email: email@example.com
Qianming Chen, email: firstname.lastname@example.org
Keywords: CypA, proteomics, oral submucous fibrosis, fibroblast
Received: December 20, 2015 Accepted: July 18, 2016 Published: August 12, 2016
Oral submucous fibrosis (OSF) is a pre-cancerous lesion, which is characterized by fibrosis of the oral submucosa. Despite large body of studies focusing on this disease, the molecular mechanisms underlying the progression of OSF remained unclear. In this study, 2-DE-based proteomic approaches were employed to identify the differently expressed proteins between OSF and normal tissues. In total, 88 proteins were identified with altered expression levels, including CypA. Upregulation of CypA was further validated through immunohistochemistry staining combined with Q-PCR and western blot by using clinical samples. Statistical analyses reveal that CypA expression level is correlated to the progression of OSF. Finally, functional study reveals a pro-proliferative property of CypA in fibroblast cells by using multiple in vitro models. The present data suggest that CypA might be a potential biomarker and therapeutic target for OSF, and will lead to a better understanding of OSF pathogenesis.
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