SC79 protects retinal pigment epithelium cells from UV radiation via activating Akt-Nrf2 signaling
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Yi-qing Gong1,2,*, Wei Huang1,*, Ke-ran Li1,*, Yuan-yuan Liu3,*, Guo-fan Cao1, Cong Cao3, Qin Jiang1
1The Affiliated Eye Hospital of Nanjing Medical University, Nanjing, China
2Ophthalmology Department, Zhenjiang First People's Hospital, Zhenjiang, China
3Institute of Neuroscience, Soochow University, Suzhou, Jiangsu, China
*These authors contributed equally to this work
Qin Jiang, email: [email protected]
Guo-fan Cao, email: [email protected]
Cong Cao, email: [email protected]
Keywords: retinal pigment epithelium, UV, SC79, Akt, Nrf2 signaling
Received: June 13, 2016 Accepted: July 20, 2016 Published: August 09, 2016
Excessive Ultra-violet (UV) radiation causes oxidative damages and apoptosis in retinal pigment epithelium (RPE) cells. Here we tested the potential activity of SC79, a novel small molecule activator of Akt, against the process. We showed that SC79 activated Akt in primary and established (ARPE-19 line) RPE cells. It protected RPE cells from UV damages possibly via inhibiting cell apoptosis. Akt inhibition, via an Akt specific inhibitor (MK-2206) or Akt1 shRNA silence, almost abolished SC79-induced RPE cytoprotection. Further studies showed that SC79 activated Akt-dependent NF-E2-related factor 2 (Nrf2) signaling and inhibited UV-induced oxidative stress in RPE cells. Reversely, Nrf2 shRNA knockdown or S40T mutation attenuated SC79-induced anti-UV activity. For the in vivo studies, we showed that intravitreal injection of SC79 significantly protected mouse retina from light damages. Based on these results, we suggest that SC79 protects RPE cells from UV damages possibly via activating Akt-Nrf2 signaling axis.
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