Research Papers: Pathology:

Deciphering bacterial community changes in zucker diabetic fatty rats based on 16S rRNA gene sequences analysis

Chunyan Gu, Ye Yang, Hong Xiang, Shu Li, Lina Liang, Hua Sui, Libin Zhan and Xiaoguang Lu _

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Oncotarget. 2016; 7:48941-48952. https://doi.org/10.18632/oncotarget.10597

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Chunyan Gu1,*, Ye Yang1,*, Hong Xiang2, Shu Li2, Lina Liang4, Hua Sui4, Libin Zhan1,2 and Xiaoguang Lu3

1 Basic Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China

2 The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China

3 Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, Liaoning, China

4 Institute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, China

* These authors contributed equally to this work

Correspondence to:

Libin Zhan, email:

Xiaoguang Lu, email:

Keywords: type 2 diabetes; zucker diabetic fatty rat; gastrointestinal microbe; 16S rRNA; microbial communities; Pathology Section

Received: January 16, 2016 Accepted: June 17, 2016 Published: July 13, 2016


The aim of the present pilot study was deciphering bacterial community changes in Zucker diabetic fatty rats (ZDF rats), a model of type 2 diabetes. Recent studies unmasked that the status of gastrointestinal tract microbiota has a marked impact on nutrition-related syndromes such as obesity and type-2 diabetes (T2D). In this study, samples taken from the gastrointestinal tracts (GI tracts) of ZDF and their lean littermates (ZL rats) were subjected to 16S rRNA gene sequence-based analysis to examine the characteristic bacterial communities, including those located in the stomach, duodenum, jejunum, ileum, cecum and feces. Results revealed that the Firmicutes/Bacteroidetes ratio was increased and greater numbers of Lactobacillus were detected along GI tracts in ZDF rats compared to ZL rats. In conclusion, this work is the first study to systematically characterize bacterial communities along ZDF rat GI tract and provides substantial evidence supporting a prospective strategy to alter the GI microbial communities improving obesity and T2D.

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