Oncotarget

Research Papers:

Triptolide inhibits cell growth and GRP78 protein expression but induces cell apoptosis in original and radioresistant NPC cells

Chengmin Li, Bin Zhang, Wuwu Lv, Chen Lai, Zhikang Chen, Ran Wang, Xueying Long and Xueping Feng _

PDF  |  HTML  |  How to cite  |  Order a Reprint

Oncotarget. 2016; 7:49588-49596. https://doi.org/10.18632/oncotarget.10412

Metrics: PDF 1290 views  |   HTML 1848 views  |   ?  


Abstract

Chengmin Li1,*, Bin Zhang2,*, Wuwu Lv1, Chen Lai3, Zhikang Chen3, Ran Wang3, Xueying Long4, Xueping Feng1

1Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, China

2Department of Histology and Embryology, Xiangya School of Medicine, Central South University, Changsha, Hunan Province 410008, China

3Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, China

4Department of Radiology, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, China

*These authors contributed equally to this work

Correspondence to:

Xueping Feng, email: fxp1029@aliyun.com

Keywords: nasopharyngeal carcinoma, CNE2, radioresistance, triptolide, GRP78

Received: November 05, 2015     Accepted: June 16, 2016     Published: July 06, 2016

ABSTRACT

The radioresistance is the key factor to hamper curative effect and survival of nasopharyngeal carcinoma (NPC) patients. Nature triptolide (TPL) has been found to circumvent drug-resistant effect of cancer, but its effect on NPC radioresistance has been rarely studied. In the present study, the 10 Gy-resistant CNE2 subclones (CNE2-SR) were used as a NPC radioresistant model. The IC50 of TPL in CNE2 and CNE2-SR cells was measured by MTT assay, cell cycle was analyzed by flow cytometry, and protein expression was examined by western blot. Our data showed that TPL treatment decreased the percentage of viable cells, and IC50 value in CNE2 and CNE2-SR cells was 23.6 ± 1.41 nmol/L and 31.2 ± 1.16 nmol/L, respectively. Six Gy was a moderate dosage of X-ray for CNE2, and 25 nM TPL was close to IC50 value of CNE2 and CNE2-SR. Six Gy X-ray and/or 25 nM TPL significantly inhibited tumor growth in nude mice. Furthermore, 6 Gy X-ray and/or 25 nM TPL significantly inhibited cell growth and induced cell apoptosis and M/G2 phase arrest in CNE2 and CNE2-SR cells. Moreover, TPL treatment significantly inhibited the expression of GRP78 protein in CNE2 and CNE2-SR cells. These results suggest that TPL may serve as a potential radiosensitizer agent for NPC treatment.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 10412