Oncotarget

Research Papers:

Preparation study of indocyanine green-rituximab: A new receptor-targeted tracer for sentinel lymph node in breast cancer

Bin-Bin Cong, Xiao Sun, Xian-Rang Song, Yan-Bing Liu, Tong Zhao, Xiao-Shan Cao, Peng-Fei Qiu, Chong-Lin Tian, Jin-Ming Yu and Yong-Sheng Wang _

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Oncotarget. 2016; 7:47526-47535. https://doi.org/10.18632/oncotarget.10204

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Abstract

Bin-Bin Cong1,2, Xiao Sun2, Xian-Rang Song3, Yan-Bing Liu2, Tong Zhao2, Xiao-Shan Cao1,2, Peng-Fei Qiu2, Chong-Lin Tian1,2, Jin-Ming Yu4,*, Yong-Sheng Wang2,*

1School of Medicine and Life Sciences, University of Jinan and Shandong Academy of Medical Sciences, Jinan, 250200, China

2Breast Cancer Center, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China

3Basic Laboratory, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China

4Radiotherapy Department, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China

*These authors contributed equally to this work

Correspondence to:

Yong-Sheng Wang, email: [email protected]

Jin-Ming Yu, email: [email protected]

Keywords: sentinel lymph node biopsy, synthetic imaging agent, rituximab, indocyanine green, lymphoscintigraphy

Received: February 22, 2016     Accepted: June 07, 2016     Published: June 21, 2016

ABSTRACT

An appropriate receptor-targeted tracer for sentinel lymph node biopsy (SLNB) was prepared. We combined the fluorescence tracer (Indocyanine green, ICG) with Rituximab (a chimeric human/murine monoclonal antibody targeting the CD20 antigen on the surface of lymphocyte) directly to produce a new tracer (ICG-Rituximab). When the new tracer drains to the lymph node, Rituximab will combine with CD20 receptor on the B-cell surface in the lymph node. If the statue of antibody-receptor connection does not reach saturation, the number of Rituximab is less than CD20. With this appropriate injection dose, the new tracer could only stay in sentinel lymph node (SLN) and make it imaging. Positive fluorescence SLN was detected 12 minutes after injection with no other organs imaging. The imaging of SLN was stable and clear for 20–24 hours. Due to SLN stained with more ICG than the lymphatic vessel, the fluorescence situation of SLN would be brighter than the vessel. The surgeon can detect the positive fluorescence SLN easily without following the fluorescence imaging lymphatic vessel. The results of our preliminary study showed that the new tracer might be useful for improving SLN imaging and worth further clinical study. SLNB with the new tracer could be a convenient method for detecting SLN and would become a standard performance in clinical practice.


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