Transcriptome sequencing of neurologic diseases associated genes in HHV-6A infected human astrocyte
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Qing Shao1,2,*, Zhe Lin1,*, Xiaohui Wu3,*, Junwei Tang4, Shuai Lu1, Dongju Feng1, Ci Cheng1, Lanqun Qing1, Kun Yao1, Yun Chen1
1Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu Province, People’s Republic of China
2Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, People’s Republic of China
3Genetic Data Analysis Group, Shanghai Biotechnology Corporation, Shanghai, People’s Republic of China
4Liver Transplantation Center of The First Affiliated Hospital and Collaborative Innovation Center For Cancer Personalized Medicine, Nanjing Medical University, Nanjing, Jiangsu Province, People’s Republic of China
*These authors contributed equally to this work
Yun Chen, email: [email protected]
Keywords: HHV-6, astrocyte, transcriptome sequencing study, neurologic diseases
Received: December 20, 2015 Accepted: June 01, 2016 Published: June 17, 2016
Human Herpesvirus 6 (HHV-6) has been involved in the development of several central nervous system (CNS) diseases, such as Alzheimer’s disease, multiple sclerosis and glioma. In order to identify the pathogenic mechanism of HHV-6A infection, we carried out mRNA-seq study of human astrocyte HA1800 cell with HHV-6A GS infection. Using mRNA-seq analysis of HA1800-control cells with HA1800-HHV-6A GS cells, we identified 249 differentially expressed genes. After investigating these candidate genes, we found seven genes associated with two or more CNS diseases: CTSS, PTX3, CHI3L1, Mx1, CXCL16, BIRC3, and BST2. This is the first transcriptome sequencing study which showed the significant association of these genes between HHV-6A infection and neurologic diseases. We believe that our findings can provide a new perspective to understand the pathogenic mechanism of HHV-6A infection and neurologic diseases.
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