Oncotarget

Research Papers:

Chromatin remodeling gene AT-rich interactive domain-containing protein 1A suppresses gastric cancer cell proliferation by targeting PIK3CA and PDK1

Qian Zhang _, Hai-Bo Yan, Jie Wang, Shu-Jian Cui, Xiao-Qing Wang, Ying-Hua Jiang, Li Feng, Peng-Yuan Yang and Feng Liu

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Oncotarget. 2016; 7:46127-46141. https://doi.org/10.18632/oncotarget.10060

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Abstract

Qian Zhang1,*, Hai-Bo Yan1,*, Jie Wang1, Shu-Jian Cui4, Xiao-Qing Wang1, Ying-Hua Jiang1, Li Feng1, Peng-Yuan Yang1,2, Feng Liu1,3

1Minhang Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai 201199, China

2Department of Systems Biology for Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China

3Department of Chemistry, Fudan University, Shanghai 200433, China

4College of Bioscience and Biotechnology, Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China

*These authors have contributed equally to this work

Correspondence to:

Feng Liu, email: liuf@fudan.edu.cn

Peng-Yuan Yang, email: pyyang@fudan.edu.cn

Keywords: ARID1A, SWI/SNF complex, gastric cancer, PIK3CA, PDK1

Received: September 16, 2015     Accepted: June 01, 2016     Published: June 15, 2016

ABSTRACT

The tumor suppressor gene AT-rich interactive domain-containing protein 1A (ARID1A) was frequently mutated in cancers. The modulation mechanism of ARID1A for PI3K/AKT signaling in gastric cancer (GC) remains elusive. Here, we found that depletion of endogenous ARID1A enhanced the in vitro proliferation, colony formation, cellular growth, nutrient uptake and in vivo xenograft tumor growth of GC cells. PI3K/AKT activation by ARID1A-silencing was profiled using a phospho-protein antibody array. The phosphorylation of PDK1, AKT, GSK3β and 70S6K, and the protein and mRNA expressions of PI3K and PDK1, were upregulated by ARID1A-silencing. Chromatin immunoprecipitation and luciferase reporter assay revealed that ARID1A-involved SWI/SNF complex inhibited PIK3CA and PDK1 transcription by direct binding to their promoters. Serial deletion mutation analyses revealed that the ARID1A central region containing the HIC1-binding domain, but not the ARID DNA-binding domain and the C-terminal domain, was essential for the inhibition of GC cell growth, PI3K/AKT pathway phosphorylation and its transcriptional modulation activity of PIK3CA and PDK1. The proliferation, cellular growth and glucose consumption of ARID1A-deficient GC cells were efficiently prohibited by allosteric inhibitors mk2206 and LY294002, which targeting AKT and PI3K, respectively. Both inhibitors also downregulated the phosphorylation of PI3K/AKT pathway in ARID1A-deficient GC cells. Such cells were sensitized to the treatment of LY294002, and AT7867, another inhibitor of AKT and p70S6K. The administration of LY294002 alone inhibited the in vivo growth of ARID1A- deficient GC cells in mouse xenograft model. Our study provides a novel insight into the modulatory function and mechanism of ARID1A in PI3K/AKT signaling in GC.


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