MicroRNA-187 inhibits tumor growth and invasion by directly targeting CD276 in colorectal cancer
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Zheng-Shi Wang1,*, Ming Zhong1,*, Yu-Hai Bian1, Yi-Fei Mu1, Shao-Lan Qin1, Min-Hao Yu1, Jun Qin1
1Department of Gastrointestinal Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China
*These authors have contributed equally to this work
Jun Qin, email: firstname.lastname@example.org
Min-Hao Yu, email: email@example.com
Keywords: microRNA-187, CD276, colorectal cancer, growth, invasion
Received: October 14, 2015 Accepted: May 11, 2016 Published: June 14, 2016
Aberrantly expressed microRNAs contribute to the initiation and progression of human cancers. However, the underlying functions of microRNA-187 (miR-187) in colorectal cancer (CRC) remain largely unexplored. Here, we demonstrated that miR-187 was significantly down-regulated in CRC tissues and cell lines compared to their normal counterparts. By Kaplan-Meier analysis, we revealed that decreased miR-187 expression was closely associated with shorter overall survival and relapse-free survival of patients with CRC. By gain- and loss-of-function studies, we showed that miR-187 remarkably suppressed CRC cell proliferation, migration, invasion, and promoted cell apoptosis. Furthermore, bioinformatics analysis and luciferase reporter assay identified that CD276 was the direct functional target of miR-187 in CRC. Genetic silencing of CD276 recapitulated similar phenotype as observed in over-expression of miR-187, and restoration of CD276 completely rescued the inhibitory effect of miR-187 in CRC cells. Taken together, our study implied the essential roles of miR-187 in suppressing CRC progression, and a novel link between miR-187 and CD276 in CRC.
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