PTRF/Cavin-1 decreases prostate cancer angiogenesis and lymphangiogenesis
Metrics: PDF 1750 views | HTML 1905 views | ?
Zeyad D. Nassar1, Hyeongsun Moon2, Tam Duong3, LiQi Neo1, Michelle M. Hill2, Mathias Francois3, Robert G. Parton3, Marie-Odile Parat1
1 The University of Queensland, School of Pharmacy, QLD, Australia.
2 The University of Queensland Diamantina Institute, Translational Research Institute, QLD, Australia.
3 The University of Queensland, Institute for Molecular Bioscience, QLD, Australia.
Marie-Odile Parat, email:
Keywords: PTRF, Caveolae, Angiogenesis, Lymphangiogenesis, Prostate Cancer
Received: August 19, 2013 Accepted: October 1, 2013 Published: October 4, 2013
Caveolae are specialized plasma membrane subdomains implicated in cellular functions such as migration, signalling and trafficking. Caveolin-1 and polymerase I and transcript release factor (PTRF)/cavin-1 are essential for caveola formation. Caveolin-1 is overexpressed and secreted in prostate tumors and promotes aggressiveness and angiogenesis. In contrast, a lack of PTRF expression is reported in prostate cancer, and ectopic PTRF expression in prostate cancer cells inhibits tumor growth and metastasis. We experimentally manipulated PTRF expression in three prostate cancer cell lines, namely the caveolin-1 positive cells PC3 and DU145 and the caveolin-1-negative LNCaP cells, to evaluate angiogenesis- and lymphangiogenesis-regulating functions of PTRF. We show that the conditioned medium of PTRF-expressing prostate cancer cells decreases ECs proliferation, migration and differentiation in vitro and ex vivo. This can occur independently from caveolin-1 expression and secretion or caveola formation, since the anti-angiogenic effects of PTRF were detected in caveolin-1-negative LNCaP cells. Additionally, PTRF expression in PC3 cells significantly decreased blood and lymphatic vessel densities in orthotopic tumors in mice. Our results suggest that the absence of PTRF in prostate cancer cells contributes significantly to tumour progression and metastasis by promoting the angiogenesis and lymphangiogenesis potential of the cancer cells, and this could be exploited for therapy.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.