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Graded expression of microRNA-371a-3p in tumor tissues, contralateral testes, and in serum


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2020-05-04

Volume 11, Number 16 of @Oncotarget reported that Mi R-levels in GCT tissue are 399-fold higher than in contralateral testicular tissue and 5843-fold higher than in non-testicular tissue.

Low expression is also found in normal testicular tissue but not in non-testicular tissue.

Mi R-371a-3p levels in tissue and serum correlate significantly.

Expression levels of mi R-371a-3p were concurrently measured in tissues of GCT, contralateral testes, and in serum with real-time PCR.

Dr. Gazanfer Belge from The Faculty of Biology and Chemistry at The University of Bremen in Bremen Germany said, "Serum levels of microRNAs (miRs) of the clusters miR-371-373 and miR-302/367 have been suggested as novel biomarkers of testicular germ cell tumors (GCTs)"

"Serum levels of microRNAs (miRs) of the clusters miR-371-373 and miR-302/367 have been suggested as novel biomarkers of testicular germ cell tumors (GCTs)."

- Dr. Gazanfer Belge, The Faculty of Biology and Chemistry at The University of Bremen

A study using RNA extraction from formalin-fixed paraffin-embedded GCT tissue again demonstrated the presence of mi R-371a-3p in tumor tissue with different expression levels in the various histological subtypes of GCT.

The only study to date” that evaluated both tissue expression levels and corresponding serum levels did not find a clear correlation between these levels.

The second goal was to explore if increasing levels of mi R371 in GCT tissue would translate into higher serum levels of the mi R.

Figure 1: Individual results of measuring miR-371a-3p expressions in GCT tumor tissue samples (dark grey) and the corresponding contralateral testicle (light grey). n = 38. The patient ID is identical with data sets in Table 1. The y-axis is displayed in a logarithmic scale.

The Belge Research Team concluded in their Oncotarget Research Article, "the present evaluation confirms the understanding that circulating miR-371a-3p-are specifically derived from cells of testicular germ cell neoplasms.

This miR, thus represents a specific tumor marker for GCTs, which is not expressed in other diseases. By contrast, the specificity of the classical tumor marker AFP is considerably hampered by its association with non-GCT related conditions, such as liver diseases"

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DOI - https://doi.org/10.18632/oncotarget.27565

Full text - https://www.oncotarget.com/article/27565/text/

Correspondence to - Gazanfer Belge - belge@uni-bremen.de

Keywords - testicular germ cell tumors, microRNA-371a-3p, serum, contralateral testes, in situ hybridization

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