Immunotherapy of acute leukemia by chimeric antigen receptor-modified lymphocytes using an improved Sleeping Beauty transposon platform
Metrics: PDF 2711 views | HTML 3796 views | ?
Chiara F. Magnani1, Nice Turazzi1, Fabrizio Benedicenti2, Andrea Calabria2, Erika Tenderini2, Sarah Tettamanti1, Greta M.P. Giordano Attianese1,3, Laurence J.N. Cooper3, Alessandro Aiuti2, Eugenio Montini2, Andrea Biondi1, Ettore Biagi1
1Tettamanti Research Center, Department of Pediatrics, University of Milano-Bicocca, San Gerardo Hospital/Fondazione MBBM, Monza, Italy
2San Raffaele Telethon Institute for Gene Therapy (HSR-TIGET), Milan, Italy
3University of Texas MD Anderson Cancer Center, Houston, Texas, USA
Andrea Biondi, email: [email protected]
Ettore Biagi, email: [email protected]
Keywords: chimeric antigen receptor, Sleeping Beauty transposon, cytokine-induced killer cells, acute lymphoblastic leukemia, acute myelogenous leukemia
Received: February 11, 2016 Accepted: May 20, 2016 Published: June 13, 2016
Chimeric antigen receptor (CAR)-modified T-cell adoptive immunotherapy is a remarkable therapeutic option proven effective in the treatment of hematological malignancies. In order to optimize cell manufacturing, we sought to develop a novel clinical-grade protocol to obtain CAR-modified cytokine-induced killer cells (CIKs) using the Sleeping Beauty (SB) transposon system. Administration of irradiated PBMCs overcame cell death of stimulating cells induced by non-viral transfection, enabling robust gene transfer together with efficient T-cell expansion. Upon single stimulation, we reached an average of 60% expression of CD123- and CD19- specific 3rd generation CARs (CD28/OX40/TCRzeta). Furthermore, modified cells displayed persistence of cell subsets with memory phenotype, specific and effective lytic activity against leukemic cell lines and primary blasts, cytokine secretion, and proliferation. Adoptive transfer of CD123.CAR or CD19.CAR lymphocytes led to a significant anti-tumor response against acute myelogenous leukemia (AML) and acute lymphoblastic leukemia (ALL) disseminated diseases in NSG mice. Notably, we found no evidence of integration enrichment near cancer genes and transposase expression at the end of the differentiation. Taken all together, our findings describe a novel donor-derived non-viral CAR approach that may widen the repertoire of available methods for T cell-based immunotherapy.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.