Oncotarget

Research Papers:

CD47 deficiency in tumor stroma promotes tumor progression by enhancing angiogenesis

Lu Gao, Kexin Chen, Qi Gao, Xiaodan Wang, Jian Sun and Yong-Guang Yang _

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Oncotarget. 2017; 8:22406-22413. https://doi.org/10.18632/oncotarget.9899

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Abstract

Lu Gao1, Kexin Chen1, Qi Gao1, Xiaodan Wang1, Jian Sun1, Yong-Guang Yang1,2

1The First Hospital and Institute of Immunology, Jilin University, Changchun, China

2Columbia Center for Translational Immunology, Columbia University College of Physicians and Surgeons, New York, New York, USA

Correspondence to:

Jian Sun, email: sunjianemail@126.com

Yong-Guang Yang, email: yy2324@columbia.edu

Keywords: angiogenesis, CD47, SIRPα, TSP1, tumorigenesis

Received: April 11, 2016     Accepted: May 20, 2016     Published: June 07, 2016

ABSTRACT

CD47 is a transmembrane protein that functions as a receptor for thrombospondin-1 (TSP1) and a ligand for inhibitory receptor signal-regulatory protein-α (SIRPα). Blocking the interaction between CD47 on tumor cells and SIRPα on macrophages has been shown to induce antitumor responses. Here we investigated the role of CD47 expression in tumor stroma in tumorigenesis by comparing tumor growth in wild-type (WT) and CD47-deficient mice after subcutaneous injection of syngeneic prostate cancer cells. We found that CD47 deficiency in tumor stromal endothelial cells enhances angiogenesis, leading to suppressed tumor necrosis formation and accelerated tumor progression. Tumors from CD47-deficient mice also showed improved vascular integrity and stability, as well as increased expression of vascular endothelial growth factor (VEGF)-A and VEGF receptor 2 (VEGFR2) compared to those from WT mice. Moreover, reduced macrophage recruitment, likely due to decreased TSP1 production, was detected in tumors from CD47-deficient mice. Our results indicate that although treatment with antibody against CD47 induces antitumor immune responses by blocking the inhibitory CD47-SIRPα signaling, this treatment may also potentially promote tumor progression by blocking CD47 signaling in tumor stromal endothelial cells.


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