Oncotarget

Research Papers: Pathology:

Tumor specific mutations in TERT promoter and CTNNB1 gene in hepatitis B and hepatitis C related hepatocellular carcinoma

Francesca Pezzuto, Francesco Izzo, Luigi Buonaguro, Clorinda Annunziata, Fabiana Tatangelo, Gerardo Botti, Franco M. Buonaguro and Maria Lina Tornesello _

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Oncotarget. 2016; 7:54253-54262. https://doi.org/10.18632/oncotarget.9801

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Abstract

Francesca Pezzuto1, Francesco Izzo2, Luigi Buonaguro1, Clorinda Annunziata1, Fabiana Tatangelo3, Gerardo Botti3, Franco M. Buonaguro1 and Maria Lina Tornesello1

1 Molecular Biology and Viral Oncology Unit, Istituto Nazionale Tumori “Fondazione G Pascale” - IRCCS, Napoli, Italy

2 Hepatobiliary Surgery Unit, Istituto Nazionale Tumori “Fondazione G Pascale” - IRCCS, Napoli, Italy

3 Department of Pathology, Istituto Nazionale Tumori “Fondazione G Pascale” – IRCCS Napoli, Italy

Correspondence to:

Maria Lina Tornesello, email:

Keywords: hepatocellular carcinoma; TERT promoter; CTNNB1; hepatitis virus; Pathology Section

Received: May 16, 2016 Accepted: May 25, 2016 Published: June 02, 2016

Abstract

Recurrent somatic mutations in the promoter region of telomerase reverse transcriptase (TERT) gene and in the exon 3 of CTNNB1 gene have been recognized as common events in hepatocellular carcinoma (HCC) with variable frequencies depending on etiology and geographical region. We have analyzed TERT promoter and CTNNB1 gene mutations in 122 cases of hepatitis B (HBV) and hepatitis C (HCV) related HCCs, in 7 cases of cholangiocarcinoma (CC) and hepatocholangiocarcinoma (HCC-CC) as well as in autologous cirrhotic tissues. Overall, 50.4% and 26% of HCC as well as 14.3% and none of CC and HCC-CC were mutated in TERT promoter and in CTNNB1 exon 3, respectively. TERT and CTNNB1 mutations were found more frequently in HCV related (53.6% and 26.4%, respectively) than HBV related (41.7% and 16.7%, respectively) HCCs and coexisted in 57.6% of CTNNB1 mutated tumors. Mutations in TERT and CTNNB1 were not associated with the functional promoter polymorphism rs2853669. No mutations were detected in the 129 non-HCC cirrhotic tissues. In conclusion, mutations in TERT promoter and in CTNNB1 gene represent specific cancer signatures in the pathogenesis of viral related HCC and could be promising early biomarkers as well as targets for tailored therapies.


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