Research Papers: Gerotarget (Focus on Aging):

Conditioned medium derived from rat amniotic epithelial cells confers protection against inflammation, cancer, and senescence

Clara Di Germanio, Michel Bernier, Michael Petr, Mauro Mattioli, Barbara Barboni _ and Rafael de Cabo

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Oncotarget. 2016; 7:39051-39064. https://doi.org/10.18632/oncotarget.9694

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Clara Di Germanio1,2, Michel Bernier2, Michael Petr2, Mauro Mattioli1,3, Barbara Barboni1,* and Rafael de Cabo2,*

1 Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy

2 Translational Gerontology Branch, National Institute on Aging, NIH, Baltimore, MD, USA

3 Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy

Correspondence to:

Barbara Barboni, email:

Rafael de Cabo, email:

Keywords: amniotic epithelial cells, inflammation, tumorigenesis, senescence, SASP, Gerotarget

Received: February 05, 2016 Accepted: May 13, 2016 Published: May 29, 2016


Amniotic epithelial cells (AECs) are a class of fetal stem cells that derives from the epiblast and resides in the amnion until birth. AECs are suitable candidates for regenerative medicine because of the ease of collection, their low immunogenicity and inability to form tumors after transplantation. Even though human AECs have been widely investigated, the fact remains that very little is known about AECs isolated from rat, one of the most common animal models in medical testing. In this study, we showed that rat AECs retained stemness properties and plasticity, expressed the pluripotency markers Sox2, Nanog, and Oct4 and were able to differentiate toward the osteogenic lineage. The addition of conditioned medium collected from rat AECs to lipopolysaccharide-activated macrophages elicited anti-inflammatory properties through a decrease of Tnfa expression and slowed tumor cell proliferation in vitro and in vivo. The senescence-associated secretory phenotype was also significantly lower upon incubation of senescent human IMR-90 fibroblast cells with conditioned medium from rat AECs. These results confirm the potential of AECs in the modulation of inflammatory mechanisms and open new therapeutic possibilities for regenerative medicine and anti-aging therapies as well.

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