Research Papers:

Activin B promotes endometrial cancer cell migration by down-regulating E-cadherin via SMAD-independent MEK-ERK1/2-SNAIL signaling

Siyuan Xiong, Christian Klausen, Jung-Chien Cheng and Peter C.K. Leung _

PDF  |  HTML  |  Supplementary Files  |  How to cite

Oncotarget. 2016; 7:40060-40072. https://doi.org/10.18632/oncotarget.9483

Metrics: PDF 1777 views  |   HTML 2355 views  |   ?  


Siyuan Xiong1, Christian Klausen1, Jung-Chien Cheng1, Peter C.K. Leung1

1Department of Obstetrics and Gynaecology, Child & Family Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada

Correspondence to:

Peter C.K. Leung, email: [email protected]

Keywords: activin B, E-cadherin, ERK1/2, cell migration, serous endometrial cancer

Received: December 19, 2015    Accepted: April 24, 2016    Published: May 19, 2016


High-risk type II endometrial cancers account for ~30% of cases but ~75% of deaths due, in part, to their tendency to metastasize. Histopathological studies of type II endometrial cancers (non-endometrioid, mostly serous) suggest overproduction of activin B and down-regulation of E-cadherin, both of which are associated with reduced survival. Our previous studies have shown that activin B increases the migration of type II endometrial cancer cell lines. However, little is known about the relationship between activin B signaling and E-cadherin in endometrial cancer. We now demonstrate that activin B treatment significantly decreases E-cadherin expression in both a time- and concentration-dependent manner in KLE and HEC-50 cell lines. Interestingly, these effects were not inhibited by knockdown of SMAD2, SMAD3 or SMAD4. Rather, the suppressive effects of activin B on E-cadherin were mediated by MEK-ERK1/2-induced production of the transcription factor SNAIL. Importantly, activin B-induced cell migration was inhibited by forced-expression of E-cadherin or pre-treatment with the activin/TGF-β type I receptor inhibitor SB431542 or the MEK inhibitor U0126. We have identified a novel SMAD-independent pathway linking enhanced activin B signaling to reduced E-cadherin expression and increased migration in type II endometrial cancer.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 9483