Research Papers:

Pseudomonas aeruginosa mannose-sensitive hemagglutinin inhibits proliferation and invasion via the PTEN/AKT pathway in HeLa cells

Tie-qiu Yin _, Xuan Ou-yang, Fang-yan Jiao, Lu-ping Huang, Xu-dong Tang and Bi-qiong Ren

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Oncotarget. 2016; 7:37121-37131. https://doi.org/10.18632/oncotarget.9467

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Tie-qiu Yin1, Xuan Ou-yang1, Fang-yan Jiao1, Lu-ping Huang1, Xu-dong Tang1, Bi-qiong Ren1

1The Second People’s Hospital of Hunan Province, Clinical Medical College of Hunan University of Chinese Medicine, Changsha 410007, China

Correspondence to:

Tie-qiu Yin, email: [email protected]

Keywords: cervical cancer, PA-MSHA, PTEN, p-AKT, p-GSK3β

Received: September 30, 2015     Accepted: April 24, 2016     Published: May 19, 2016


We investigated the effects of Pseudomonas aeruginosa mannose-sensitive hemagglutinin (PA-MSHA) on the proliferation and invasion of human cervical cancer cell lines, as well as the molecular pathways underlying these effects. MTT cell proliferation assays revealed a time- and concentration-dependent cytotoxic effect of PA-MSHA on HeLa cells but not H8 cells. Flow cytometry with propidium iodide and annexin-V-fluorescein isothiocyanate labeling (FITC) indicated that various concentrations of PA-MSHA could induce apoptosis and G2-M cell cycle arrest in HeLa cells. PA-MSHA also impaired the migration and invasion abilities of HeLa cells in Wound healing and Transwell invasion assays. Western blot results demonstrated that PA-MSHA reduced the expression of p-AKT, p-GSK3β, BCL-2, Vimentin and β-catenin, but increased the levels of PTEN, BAD, BAX and E-cadherin in HeLa cells. Importantly, PTEN siRNA induced the activity of p-AKT, while PA-MSHA partly inhibited this induction, indicating that PA-MSHA may reduce the cell proliferation and invasion potential by activating PTEN and thus inhibiting the AKT pathway in vitro. These data suggest the potential application of PA-MSHA to the treatment of human cervical cancer.

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PII: 9467