Down-regulation of ABCG2 and ABCB4 transporters in the placenta of rats exposed to cadmium
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Lili Liu1,2, Liang Zhou1, Shuiwang Hu3, Shanyu Zhou2, Yingyu Deng2, Ming Dong2, Jianxun Huang2, Yuli Zeng2, Xiaoyan Chen2, Na Zhao2, Hongling Li2, Zhenhua Ding1
1Department of Radiation Medicine, School of Public Health and Tropic Medicine, Southern Medical University, Guangzhou, China
2Department of Toxicology, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, China
3Department of Pathophysiology, Southern Medical University, Guangzhou, China
Zhenhua Ding, email: [email protected]
Keywords: cadmium, proteomics, placenta, ABCG2, ABCB4
Received: March 19, 2016 Accepted: April 27, 2016 Published: May 17, 2016
As a maternal and developmental toxicant, cadmium (Cd) possesses weak penetrability through the placental barrier. However, the underlying mechanism remains unclear. To gain insight into the protein molecules associated with Cd toxicity in placenta and explore their roles in Cd transportation, a reproductive animal experiment was carried out using Sprague-Dawley rats. We performed proteomic analysis of the placenta by Difference Gel Electrophoresis (DIGE) combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Tandem Mass Spectroscopy (MALDI-TOF/TOF MS). The DIGE assay identified 15 protein spots that were differentially expressed with a greater than 1.5-fold change in placenta of Cd-treated rats compared to the control rats. Based on the expression patterns and biological functions of the proteins, we selected the ABCG2 and ABCB4 transporter proteins for further analysis. Western blot analysis showed that Cd exposure could down-regulate the expression of ABCG2 and ABCB4 in the placenta. There was a negative dose-response relationship between Cd exposure and the expression of ABCG2 or ABCB4 protein. These results indicated that down-regulation of ABCG2 and ABCB4 transporters may regulate Cd across through placenta and thus affect the in vivo toxic effect of Cd to fetus.
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