Research Papers:

Isolation, identification, and characterization of novel nanovesicles

Huang-Ge Zhang _, Pengxiao Cao, Yun Teng, Xin Hu, Qilong Wang, Ashish S. Yeri, Xiaoying Zhuang, Abhilash Samykutty, Jingyao Mu, Zhong-Bin Deng, Lifeng Zhang, James A. Mobley, Jun Yan, Kendall Van Keuren-Jensen and Donald Miller

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Oncotarget. 2016; 7:41346-41362. https://doi.org/10.18632/oncotarget.9325

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Huang-Ge Zhang1,2, Pengxiao Cao2, Yun Teng2, Xin Hu4,5, Qilong Wang2,8, Ashish S. Yeri6, Xiaoying Zhuang2, Abhilash Samykutty2, Jingyao Mu2, Zhong-Bin Deng3, Lifeng Zhang2, James A. Mobley7, Jun Yan3, Kendall Van Keuren-Jensen6, Donald Miller3

1Louisville Veterans Administration Medical Center, Louisville, KY 40206, USA

2James Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville, KY 40202, USA

3Department of Medicine, University of Louisville, KY 40202, USA

4Program in Biostatistics, Bioinformatics and Systems Biology, The University of Texas Graduate School of Biomedical Sciences at Houston, TX 77030, USA

5Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA

6Translational Genomics Research Institute, Phoenix, AZ 85004, USA

7Mass Spectrometry/Proteomics Shared Facility, University of Alabama at Birmingham, Birmingham, AL 35294, USA

8Department of Clinical Oncology, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, 223300, China

Correspondence to:

Huang-Ge Zhang, email: H0Zhan17@louisville.edu

Keywords: isolation and identification extracellular microvesicles, in vivo predominately population, HG-NV, exosomes

Received: November 12, 2015     Accepted: April 16, 2016     Published: May 12, 2016


Extracellular microvesicles (EVs) have been recognized for many potential clinical applications including biomarkers for disease diagnosis. In this study, we identified a major population of EVs by simply screening fluid samples with a nanosizer. Unlike other EVs, this extracellular nanovesicle (named HG-NV, HG-NV stands for HomoGenous nanovesicle as well as for Huang-Ge- nanovesicle) can be detected with a nanosizer with minimal in vitro manipulation and are much more homogenous in size (8–12 nm) than other EVs. A simple filtration platform is capable of separating HG-NVs from peripheral blood or cell culture supernatants. In comparison with corresponding exosome profiles, HG-NVs released from both mouse and human breast tumor cells are enriched with RNAs. Tumor derived HG-NVs are more potent in promoting tumor progression than exosomes. In summary, we identified a major subset of EVs as a previously unrecognized nanovesicle. Tumor cell derived HG-NVs promote tumor progression. Molecules predominantly present in breast tumor HG-NVs have been identified and characterized. This discovery may have implications in advancing both microvesicle biology research and clinical management including potential used as a biomarker.

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