Isolation, identification, and characterization of novel nanovesicles
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Huang-Ge Zhang1,2, Pengxiao Cao2, Yun Teng2, Xin Hu4,5, Qilong Wang2,8, Ashish S. Yeri6, Xiaoying Zhuang2, Abhilash Samykutty2, Jingyao Mu2, Zhong-Bin Deng3, Lifeng Zhang2, James A. Mobley7, Jun Yan3, Kendall Van Keuren-Jensen6, Donald Miller3
1Louisville Veterans Administration Medical Center, Louisville, KY 40206, USA
2James Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville, KY 40202, USA
3Department of Medicine, University of Louisville, KY 40202, USA
4Program in Biostatistics, Bioinformatics and Systems Biology, The University of Texas Graduate School of Biomedical Sciences at Houston, TX 77030, USA
5Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
6Translational Genomics Research Institute, Phoenix, AZ 85004, USA
7Mass Spectrometry/Proteomics Shared Facility, University of Alabama at Birmingham, Birmingham, AL 35294, USA
8Department of Clinical Oncology, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, 223300, China
Huang-Ge Zhang, email: H0Zhan17@louisville.edu
Keywords: isolation and identification extracellular microvesicles, in vivo predominately population, HG-NV, exosomes
Received: November 12, 2015 Accepted: April 16, 2016 Published: May 12, 2016
Extracellular microvesicles (EVs) have been recognized for many potential clinical applications including biomarkers for disease diagnosis. In this study, we identified a major population of EVs by simply screening fluid samples with a nanosizer. Unlike other EVs, this extracellular nanovesicle (named HG-NV, HG-NV stands for HomoGenous nanovesicle as well as for Huang-Ge- nanovesicle) can be detected with a nanosizer with minimal in vitro manipulation and are much more homogenous in size (8–12 nm) than other EVs. A simple filtration platform is capable of separating HG-NVs from peripheral blood or cell culture supernatants. In comparison with corresponding exosome profiles, HG-NVs released from both mouse and human breast tumor cells are enriched with RNAs. Tumor derived HG-NVs are more potent in promoting tumor progression than exosomes. In summary, we identified a major subset of EVs as a previously unrecognized nanovesicle. Tumor cell derived HG-NVs promote tumor progression. Molecules predominantly present in breast tumor HG-NVs have been identified and characterized. This discovery may have implications in advancing both microvesicle biology research and clinical management including potential used as a biomarker.
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